Genetic targeting of candidate genes for drought sensitive gene eibi1 of wild barley (Hordeum spontaneum)

被引:7
作者
Chen, Guoxiong [1 ,2 ]
Pourkheirandish, Mohammad [1 ]
Sameri, Mohammad [1 ]
Wang, Ning [1 ]
Nair, Sudha [1 ]
Shi, Yulan [2 ]
Li, Chao [2 ]
Nevo, Eviatar [3 ]
Komatsuda, Takao [1 ]
机构
[1] Natl Inst Agrobiol Sci, Plant Genome Res Unit, Tsukuba, Ibaraki 3058602, Japan
[2] Lanzhou Univ, Extreme Stress Resistance & Biotechnol Lab, Cold & Arid Reg Environm & Engn Inst, Lanzhou 730000, Peoples R China
[3] Univ Haifa, Inst Evolut, IL-32905 Haifa, Israel
关键词
cuticle; eibi1; mutant; synteny; wild barley; rice; fine mapping; CUTICULAR WAX; CUTICLE DEVELOPMENT; ABC TRANSPORTER; ARABIDOPSIS-THALIANA; ECERIFERUM MUTANTS; BIOSYNTHESIS; MUTATION; REVEALS; LEUNIG; POLLEN;
D O I
10.1270/jsbbs.59.637
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Drought stress is one of the most severe abiotic stresses that cause the loss of crop yield. The cuticle protects the leaf from dehydration in the face of drought stress. The barley cuticle mutant eibi1 is highly drought sensitive. Here, we describe the fine-scale genetic mapping of the eibi1 locus, based on a cv. Morex x eibi1 F-2 population of 1,682 individuals. Barley-rice synteny was exploited to identify markers for mapping and to identify candidate genes for eibi1. The target segment of chromosome 3H is perfectly collinear with the equivalent region on rice chromosome 1. Marker enrichment delimited eibi1 to a 0.11 cM barley region defined by the interval B1958842-Os01g0176800*, which in rice consists of a 112.8 kbp segment. Gene prediction revealed that this rice segment harbours 16 genes. Of them, five (Os01g0177100, Os01g0177200, Os01g0177900, Os01g0178200 and Os01g0178400) were proposed as candidate genes of Eibi1.
引用
收藏
页码:637 / 644
页数:8
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