Semi-quantitative analysis of drugs of abuse, including tetrahydrocannabinol in hair using aqueous extraction and immunoassay

被引：20

作者：

Coulter, Cynthia ^{[1
]}

Tuyay, James ^{[1
]}

Taruc, Margaux ^{[1
]}

Moore, Christine ^{[1
]}

机构：

[1] Immunalysis Corp, Pomona, CA 91767 USA

来源：

FORENSIC SCIENCE INTERNATIONAL | 2010年 / 196卷 / 1-3期

关键词：

Hair; Drugs of abuse; Immunoassay; IMMUNALYSIS(R) MICROPLATE ELISA; VALIDATION;

D O I：

10.1016/j.forsciint.2009.12.025

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A semi-quantitative analytical screening procedure for the determination of cocaine, amphetamines, opiates, and delta-9-tetrahydrocannabinol in hair has been developed. The procedure employs an aqueous extraction buffer, uses only 10 mg of hair, requires 2 h of incubation for the extraction to occur, and multiple drug classes can be screened using enzyme linked immunosorbent assays. Hair calibration standards were prepared around the recommended cut-off concentrations of the Society of Hair Testing. All drug classes showed excellent linearity over the concentration range tested, indicating that immunochemical screening can be used in a semi-quantitative mode for hair analysis using an aqueous buffer, rapid extraction and a small amount of hair. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

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页码：70 / 73

页数：4

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