The distribution and frequency of microsatellite loci in Drosophila melanogaster

被引：106

作者：

Schug, MD ^{[1
]}

Wetterstrand, KA ^{[1
]}

Gaudette, MS ^{[1
]}

Lim, RH ^{[1
]}

Hutter, CM ^{[1
]}

Aquadro, CF ^{[1
]}

机构：

[1] Cornell Univ, Genet & Dev Sect, Ithaca, NY 14853 USA

来源：

MOLECULAR ECOLOGY | 1998年 / 7卷 / 01期

关键词：

dinucleotide repeats; Drosophila; simple sequence length polymorphism; simple sequence repeats; SSLP; SSR;

D O I：

10.1046/j.1365-294x.1998.00304.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the results of a comprehensive search of Drosophila melanogaster DNA sequences in GenBank for di-, tri-, and tetranucleotide repeats of more than four repeat units, and a DNA library screen for dinucleotide repeats. Dinucleotide repeats are more abundant (66%) than tri- (30%) or tetranucleotide (4%) repeats. We estimate that 1917 dinucleotide repeats with 10 or more repeat units are present in the euchromatic D. melanogaster genome and, on average, they occur once every 60 kb. Relative to many other animals, dinucleotide repeats in D. melanogaster are short. Tri- and tetranucleotide repeats have even fewer repeat units on average than dinucleotide repeats. Our WorldWide Web site (http://www.bio.cornell.edu/genetics/aquadro.html) posts the complete list of 1298 microsatellites (greater than or equal to five repeat units) identified from the GenBank search. We also summarize assay conditions for 70 D. melanogaster microsatellites characterized in previous studies and an additional 56 newly characterized markers.

引用

页码：57 / 70

页数：14

共 42 条

[1] Microsatellite variation in grey seals (Halichoerus grypus) shows evidence of genetic differentiation between two British breeding colonies [J].