Bio-inspired crosslinking and matrix-drug interactions for advanced wound dressings with long-term antimicrobial activity

被引：167

作者：

Dhand, Chetna ^{[1
,2
]}

Venkatesh, Mayandi ^{[1
]}

Barathi, Veluchami Amutha ^{[1
,2
,3
]}

Harini, Sriram ^{[1
]}

Bairagi, Samiran ^{[4
]}

Leng, Eunice Goh Tze ^{[1
]}

Muruganandham, Nandhakumar ^{[1
,5
]}

Low, Kenny Zhi Wei ^{[6
]}

Fazil, Mobashar Hussain Urf Turabe ^{[7
]}

Loh, Xian Jun ^{[6
]}

Srinivasan, Dinesh Kumar ^{[8
]}

Liu, Shou Ping ^{[1
,2
]}

Beuerman, Roger W. ^{[1
,2
]}

Verma, Navin Kumar ^{[1
,7
]}

Ramakrishna, Seeram ^{[4
,9
]}

Lakshminarayanan, Rajamani ^{[1
,2
]}

机构：

[1] The Academia, Singapore Eye Res Inst, Antiinfect Res Grp, 20 Coll Rd,Discovery Tower, Singapore 169856, Singapore

[2] Duke NUS Grad Med Sch, Ophthalmol & Visual Sci Acad Clin Program, Singapore 169857, Singapore

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Ophthalmol, Singapore 119077, Singapore

[4] Natl Univ Singapore, Dept Mech Engn, Ctr Nanofibers & Nanotechnol, Fac Engn, 2 Engn Dr 3, Singapore 117576, Singapore

[5] Quintiles Translat, 79 Sci Pk Dr,06-08 CINTECH 4,Sci Pk One, Singapore 118264, Singapore

[6] ASTAR, Inst Mat Res & Engn, 2 Fusionopolis Way,Innovis 08-03, Singapore 138634, Singapore

[7] Nanyang Technol Univ, Lee Kong Chian Sch Med, Expt Med Bldg, Singapore 636921, Singapore

[8] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Anat, Singapore 117594, Singapore

[9] Jinan Univ, GHMICR, Guangzhou 510632, Guangdong, Peoples R China

来源：

BIOMATERIALS | 2017年 / 138卷

基金：

英国医学研究理事会; 新加坡国家研究基金会;

关键词：

Polyhydroxy antimicrobial; Wound dressings; Polydopamine crosslinking; Gelatin; Electrospinning; IN-VITRO; ELECTROSPUN NANOFIBERS; SURFACE MODIFICATION; CONTROLLED-RELEASE; GELATIN; VANCOMYCIN; FIBERS; MECHANISMS; SCAFFOLD; GROWTH;

D O I：

10.1016/j.biomaterials.2017.05.043

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

There is a growing demand for durable advanced wound dressings for the management of persistent infections after deep burn injuries. Herein, we demonstrated the preparation of durable antimicrobial nanofiber mats, by taking advantage of strong interfacial interactions between polyhydroxy antibiotics (with varying number of -OH groups) and gelatin and their in-situ crosslinking with polydopamine (pDA) using ammonium carbonate diffusion method. Polydopamine crosslinking did not interfere with the antimicrobial efficacy of the loaded antibiotics. Interestingly, incorporation of antibiotics containing more number of alcoholic -OH groups (NOH >= 5) delayed the release kinetics with complete retention of antimicrobial activity for an extended period of time (20 days). The antimicrobials-loaded mats displayed superior mechanical and thermal properties than gelatin or pDA-crosslinked gelatin mats. Mats containing polyhydroxy antifungals showed enhanced aqueous stability and retained nanofibrous morphology under aqueous environment for more than 4 weeks. This approach can be expanded to produce mats with broad spectrum antimicrobial properties by incorporating the combination of antibacterial and antifungal drugs. Direct electrospinning of vancomycin-loaded electrospun nanofibers onto a bandage gauze and subsequent crosslinking produced non-adherent durable advanced wound dressings that could be easily applied to the injured sites and readily detached after treatment. In a partial thickness burn injury model in piglets, the drug-loaded mats displayed comparable wound closure to commercially available silver-based dressings. This prototype wound dressing designed for easy handling and with long-lasting antimicrobial properties represents an effective option for treating life-threatening microbial infections due to thermal injuries. (C) 2017 The Authors. Published by Elsevier Ltd.

引用

页码：153 / 168

页数：16

共 53 条

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Electrospun Nanofibers as Dressings for Chronic Wound Care: Advances, Challenges, and Future Prospects [J].