共 43 条
The small-subunit processome is a ribosome assembly intermediate
被引:145
作者:

Bernstein, KA
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机构: Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Gallagher, JEG
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机构: Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Mitchell, BM
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h-index: 0
机构: Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Granneman, S
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机构: Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Baserga, SJ
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h-index: 0
机构: Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
机构:
[1] Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA
[3] Yale Univ, Sch Med, Dept Therapeut Radiol, New Haven, CT 06520 USA
关键词:
D O I:
10.1128/EC.3.6.1619-1626.2004
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
The small-subunit (SSU) processome is a large ribonucleoprotein required for the biogenesis of the 18S rRNA and likely corresponds to the terminal knobs visualized by electron microscopy on the 5' end of nascent rRNAs. The original purification of the SSU processome of Saccharomyces cerevisiae resulted in the identification of 28 proteins. Here, we characterize 12 additional protein components, including five small-ribosomal-subunit proteins (Rps4, Rps6, Rps7, Rps9, and Rps14) that had previously been copurified. Our multiple criteria for including a component as a bona fide SSU processome component included coimmunoprecipitation with Mpp10 (an SSU processome component), the U3 snoRNA, and the anticipated pre-rRNAs. Importantly, the association of specific ribosomal proteins with the SSU processome suggests that the SSU processome has roles in both pre-rRNA processing and ribosome assembly. These ribosomal proteins may be analogous to the primary or secondary RNA binding proteins first described in bacterial in vitro ribosome assembly maps. In addition to the ribosomal proteins and based on the same experimental approach, we found seven other proteins (Utp18, Noc4, Utp20, Utp21, Utp22, Emg1, and Krr1) to be bona fide SSU processome proteins.
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页码:1619 / 1626
页数:8
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机构: Cellzome AG, D-69117 Heidelberg, Germany

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机构: Cellzome AG, D-69117 Heidelberg, Germany

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