The small-subunit processome is a ribosome assembly intermediate

被引:145
作者
Bernstein, KA
Gallagher, JEG
Mitchell, BM
Granneman, S
Baserga, SJ
机构
[1] Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA
[3] Yale Univ, Sch Med, Dept Therapeut Radiol, New Haven, CT 06520 USA
关键词
D O I
10.1128/EC.3.6.1619-1626.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The small-subunit (SSU) processome is a large ribonucleoprotein required for the biogenesis of the 18S rRNA and likely corresponds to the terminal knobs visualized by electron microscopy on the 5' end of nascent rRNAs. The original purification of the SSU processome of Saccharomyces cerevisiae resulted in the identification of 28 proteins. Here, we characterize 12 additional protein components, including five small-ribosomal-subunit proteins (Rps4, Rps6, Rps7, Rps9, and Rps14) that had previously been copurified. Our multiple criteria for including a component as a bona fide SSU processome component included coimmunoprecipitation with Mpp10 (an SSU processome component), the U3 snoRNA, and the anticipated pre-rRNAs. Importantly, the association of specific ribosomal proteins with the SSU processome suggests that the SSU processome has roles in both pre-rRNA processing and ribosome assembly. These ribosomal proteins may be analogous to the primary or secondary RNA binding proteins first described in bacterial in vitro ribosome assembly maps. In addition to the ribosomal proteins and based on the same experimental approach, we found seven other proteins (Utp18, Noc4, Utp20, Utp21, Utp22, Emg1, and Krr1) to be bona fide SSU processome proteins.
引用
收藏
页码:1619 / 1626
页数:8
相关论文
共 43 条
[1]   Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits [J].
Adams, CC ;
Jakovljevic, J ;
Roman, J ;
Harnpicharnchai, P ;
Woolford, JL .
RNA, 2002, 8 (02) :150-165
[2]   Degradation of ribosomal RNA precursors by the exosome [J].
Allmang, Christine ;
Mitchell, Philip ;
Petfalski, Elisabeth ;
Tollervey, David .
NUCLEIC ACIDS RESEARCH, 2000, 28 (08) :1684-1691
[3]   Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis [J].
Chen, WD ;
Bucaria, J ;
Band, DA ;
Sutton, A ;
Sternglanz, R .
NUCLEIC ACIDS RESEARCH, 2003, 31 (02) :690-699
[4]   A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis [J].
Dragon, F ;
Gallagher, JEG ;
Compagnone-Post, PA ;
Mitchell, BM ;
Porwancher, KA ;
Wehner, KA ;
Wormsley, S ;
Settlage, RE ;
Shabanowitz, J ;
Osheim, Y ;
Beyer, AL ;
Hunt, DF ;
Baserga, SJ .
NATURE, 2002, 417 (6892) :967-970
[5]   A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis [J].
Dunbar, DA ;
Dragon, F ;
Lee, SJ ;
Baserga, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (24) :13027-13032
[6]   Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast [J].
Dunbar, DA ;
Wormsley, S ;
Agentis, TM ;
Baserga, SJ .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (10) :5803-5812
[7]   Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis [J].
Eschrich, D ;
Buchhaupt, M ;
Kötter, P ;
Entian, KD .
CURRENT GENETICS, 2002, 40 (05) :326-338
[8]   Functional organization of the yeast proteome by systematic analysis of protein complexes [J].
Gavin, AC ;
Bösche, M ;
Krause, R ;
Grandi, P ;
Marzioch, M ;
Bauer, A ;
Schultz, J ;
Rick, JM ;
Michon, AM ;
Cruciat, CM ;
Remor, M ;
Höfert, C ;
Schelder, M ;
Brajenovic, M ;
Ruffner, H ;
Merino, A ;
Klein, K ;
Hudak, M ;
Dickson, D ;
Rudi, T ;
Gnau, V ;
Bauch, A ;
Bastuck, S ;
Huhse, B ;
Leutwein, C ;
Heurtier, MA ;
Copley, RR ;
Edelmann, A ;
Querfurth, E ;
Rybin, V ;
Drewes, G ;
Raida, M ;
Bouwmeester, T ;
Bork, P ;
Seraphin, B ;
Kuster, B ;
Neubauer, G ;
Superti-Furga, G .
NATURE, 2002, 415 (6868) :141-147
[9]   90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors [J].
Grandi, P ;
Rybin, V ;
Bassler, J ;
Petfalski, E ;
Strauss, D ;
Marzioch, M ;
Schäfer, T ;
Kuster, B ;
Tschochner, H ;
Tollervey, D ;
Gavin, AC ;
Hurt, E .
MOLECULAR CELL, 2002, 10 (01) :105-115
[10]   Ribosome biogenesis: of knobs and RNA processing [J].
Granneman, S ;
Baserga, SJ .
EXPERIMENTAL CELL RESEARCH, 2004, 296 (01) :43-50