Low-dose automated electron tomography: A recent implementation

被引：35

作者：

Rath, BK ^{[1
]}

Marko, M

Radermacher, M

Frank, J

机构：

[1] New York State Dept Hlth, Wadsworth Ctr Labs & Res, Albany, NY 12201 USA

[2] SUNY Albany, Dept Biomed Sci, Albany, NY 12222 USA

[3] Max Planck Inst Biophys, D-60596 Frankfurt, Germany

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1997年 / 120卷 / 03期

关键词：

automated electron tomography; transmission electron microscopy; low-dose microscopy; three-dimensional reconstruction; triad junction; IVEM;

D O I：

10.1006/jsbi.1997.3924

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made. (C) 1997 Academic Press.

引用

页码：210 / 218

页数：9

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