Stimulation of PAR-2 excites and sensitizes rat cutaneous C-nociceptors to heat

被引：12

作者：

Ding-Pfennigdorff, D ^{[1
]}

Averbeck, B ^{[1
]}

Michaelis, M ^{[1
]}

机构：

[1] Aventis Pharma Deutschland GmbH, DG Thrombosis & Degenerat Joint Dis, D-65929 Frankfurt, Germany

来源：

NEUROREPORT | 2004年 / 15卷 / 13期

关键词：

G protein-coupled receptor; hyperalgesia; mechanical stimulation; nociception; pain;

D O I：

10.1097/00001756-200409150-00015

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Proteinase-activated receptor 2 (PAR-2) is expressed on many nociceptive neurons. Application of PAR-2 agonists has been shown to induce behavioral signs of hyperalgesia. We investigated effects of the rat PAR-2 agonist SLIGRL-NH2 in the isolated rat skin-saphenous nerve preparation. SLIGRL-NH2 (100 muM) excited 20% of all C-fiber nociceptors tested. In addition, C-fiber nociceptors were sensitized to heat after SLIGRL-NH2 application resulting in an increase in response magnitude and a decrease of heat threshold. The PAR-2-inactive control peptide LRGILS-NH2 had no effect. The mechanical sensitivity of C-fibers was not affected by SLIGRL-NH2. PAR-2-mediated excitation and sensitization of primary nociceptors may contribute to PAR-2-mediated hyperalgesia.

引用

收藏

页码：2071 / 2075

页数：5

相关论文

共 23 条

[1]

Tryptase: potential role in airway inflammation and remodeling [J].

Abraham, WM .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2002, 282 (02) :L193-L196

[2]

Protease-activated receptor 2 sensitizes the capsaicin receptor transient receptor potential vanilloid receptor 1 to induce hyperalgesia [J].

Amadesi, S ;

Nie, JJ ;

Vergnolle, N ;

Cottrell, GS ;

Grady, EF ;

Trevisani, M ;

Manni, C ;

Geppetti, P ;

McRoberts, JA ;

Ennes, H ;

Davis, B ;

Mayer, EA ;

Bunnett, NW .

JOURNAL OF NEUROSCIENCE, 2004, 24 (18) :4300-4312

[3]

Activation of protease-activated receptor (PAR)-1, PAR-2, and PAR-4 stimulates IL-6, IL-8, and prostaglandin E2 release from human respiratory epithelial cells [J].

Asokananthan, N ;

Graham, PT ;

Fink, J ;

Knight, DA ;

Bakker, AJ ;

McWilliam, AS ;

Thompson, PJ ;

Stewart, GA .

JOURNAL OF IMMUNOLOGY, 2002, 168 (07) :3577-3585

[4]

SYNTHETIC INTERSTITIAL FLUID FOR ISOLATED MAMMALIAN TISSUE [J].

BRETAG, AH .

LIFE SCIENCES PART 1 PHYSIOLOGY AND PHARMACOLOGY AND PART 2 BIOCHEMISTRY GENERAL AND MOLECULAR BIOLOGY, 1969, 8 (5P1) :319-&

[5]

The capsaicin receptor: a heat-activated ion channel in the pain pathway [J].

Caterina, MJ ;

Schumacher, MA ;

Tominaga, M ;

Rosen, TA ;

Levine, JD ;

Julius, D .

NATURE, 1997, 389 (6653) :816-824

[6]

A protective role for protease-activated receptors in the airways [J].

Cocks, TM ;

Fong, B ;

Chow, JM ;

Anderson, GP ;

Frauman, AG ;

Goldie, RG ;

Henry, PJ ;

Carr, MJ ;

Hamilton, JR ;

Moffatt, JD .

NATURE, 1999, 398 (6723) :156-160

[7]

Proteinase-activated receptor 2-mediated potentiation of transient receptor potential vanilloid subfamily 1 activity reveals a mechanism for proteinase-induced inflammatory pain [J].

Dai, Y ;

Moriyama, T ;

Higashi, T ;

Togashi, K ;

Kobayashi, K ;

Yamanaka, H ;

Tominaga, M ;

Noguchi, K .

JOURNAL OF NEUROSCIENCE, 2004, 24 (18) :4293-4299

[8]

Proteinase-activated receptors:: novel mechanisms of signaling by serine proteases [J].

Déry, O ;

Corvera, CU ;

Steinhoff, M ;

Bunnett, NW .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1998, 274 (06) :C1429-C1452

[9]

FRAKI JE, 1983, BRIT J DERMATOL, V109, P72

[10]

The proteinase-activated receptor 2 is involved in nociception [J].

Hoogerwerf, WA ;

Zou, L ;

Shenoy, M ;

Sun, D ;

Micci, MA ;

Lee-Hellmich, H ;

Xiao, SY ;

Winston, JH ;

Pasricha, PJ .

JOURNAL OF NEUROSCIENCE, 2001, 21 (22) :9036-9042