Analysis of expressed sequence tags of flower buds in Lotus japonicus

被引：46

作者：

Endo, M

Kokubun, T

Takahata, Y

Higashitani, A

Tabata, S

Watanabe, M

机构：

[1] Iwate Univ, Fac Agr, Lab Plant Breeding, Morioka, Iwate 0208550, Japan

[2] Soma Agr High Sch, Haramachi 9750012, Japan

[3] Tohoku Univ, Inst Ecol Genet, Aoba Ku, Sendai, Miyagi 9808577, Japan

[4] Kazusa DNA Res Inst, Kisarazu 2920812, Japan

来源：

DNA RESEARCH | 2000年 / 7卷 / 03期

关键词：

legume; Lotus japonicus; ESTs; flower bud; pollen-specific gene;

D O I：

10.1093/dnares/7.3.213

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomly selected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant sequence similarities to known genes. Several genes encoding these EST clones were identified as pollen-specific genes, such as pectin methylesterase, ascorbate oxidase, and polygalacturonase, and as homologous genes involved in pollen-pistil interaction. Comparison of these EST sequences with those derived from the whole plant of L, japonicus, revealed that 64.8% of EST sequences from the flower buds were not found in EST sequences of the whole plant. Taken together, the EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of L. japonicus.

引用

页码：213 / 216

页数：4

共 31 条

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