Characterization of flavonoids in the traditional Chinese herbal medicine-Huangqin by liquid chromatography coupled with electrospray ionization mass spectrometry

被引：163

作者：

Han, Jian ^{[1
]}

Ye, Min ^{[1
]}

Xu, Man ^{[1
]}

Sun, Jianghao ^{[1
]}

Wang, Baorong ^{[1
]}

Guo, Dean ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 848卷 / 02期

关键词：

Huangqin; flavonoids; HPLC/DAD/ESI-MSn; Chinese medicine;

D O I：

10.1016/j.jchromb.2006.10.061

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The root of Scutellaria baicalensis, called Huangqin in Chinese, is one of the most commonly used traditional Chinese medicines for the treatment of hepatitis. tumors, diarrhea, and inflammatory diseases. The major chemical constituents of Huangqin are flavonoids. In the present paper, HPLC-DAD-ESI-MSn was used to analyze flavonoids in the roots of S. baicalensis. A total of 26 flavonoids were identified or tentatively characterized. including 5 C-glycosides, 12 O-glycosides, and 9 free aglycones. Two C-glycosides, apigenin-6-C-glucyl-8-C-arabinoside and chrysin-6.8-di-C-glucoside, together with some O-glycosides, are reported from S. baicalensis for the first time. This method is simple, reliable and sensitive. and could be used for the quality control of Huangqin and its related preparations. (c) 2006 Elsevier B.V. All rights reserved.

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收藏

页码：355 / 362

页数：8

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