Identification of early plasma cells in peripheral blood and their clinical significance

被引:92
作者
Harada, Y [1 ]
Kawano, MM [1 ]
Huang, NH [1 ]
Mahmoud, MS [1 ]
Lisukov, IA [1 ]
Mihara, K [1 ]
Tsujimoto, T [1 ]
Kuramoto, A [1 ]
机构
[1] HIROSHIMA UNIV, RES INST RADIAT BIOL & MED,DEPT HAEMATOL & ONCOL, MYLOMA STUDY GRP,MINANI KU, HIROSHIMA 734, JAPAN
关键词
plasma cells; early plasma cells; phenotypic analysis; multiple myeloma; autoimmune disease;
D O I
10.1046/j.1365-2141.1996.300835.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In the peripheral blood (PB) we detected so-called early plasma cells that might already be committed to entering the bone marrow (BM). By two-colour staining with FITC-anti-CD38 antibody, their intensity (CD38(++)) of expression of CD38 antigen was between that of germinal centre (GC) B cells (low expression (CD38(+))) and that of BM plasma cells (high expression (CD38(+++))), and their phenotype was CD38(++) CD19(+) CD10(-) CD20(-) CD21(+) CD24(-) CD39(+) CD5(-) VLA-4(+) VLA-5(-) MPC-1(-) without expression of surface membrane IgM (SmIgM). Morphological and immunological examination of the sorted cells confirmed that they were plasmacytoid cells with expression of cytoplasmic IgG (cIgG). Variations of these early plasma cells were examined in various diseases. In active systemic lupus erythematosus, bacterial septicaemia and liver cirrhosis, early plasma cell levels were significantly increased in PB, and after subsidence of such inflammation (inactive states) these cells returned to normal levels. In contrast, normal early plasma cells were significantly suppressed in myelomas, whilst normal or slightly increased numbers of early plasma cells was found in benign monoclonal gammopathy (BMG). In addition, the number of normal early plasma cells returned to a normal level in myeloma cases with complete responses, Therefore, early plasma cells were identified phenotypically, and an increase and decrease in these cells in PB may reflect mobilization and suppression, respectively, of activated B cells into RM plasma cells.
引用
收藏
页码:184 / 191
页数:8
相关论文
共 18 条
[1]   STAGES OF B-CELL DIFFERENTIATION IN HUMAN LYMPHOID-TISSUE [J].
BHAN, AK ;
NADLER, LM ;
STASHENKO, P ;
MCCLUSKEY, RT ;
SCHLOSSMAN, SF .
JOURNAL OF EXPERIMENTAL MEDICINE, 1981, 154 (03) :737-749
[2]   IDENTIFICATION OF MALIGNANT PLASMA-CELL PRECURSORS IN THE BONE-MARROW OF MULTIPLE-MYELOMA [J].
CALIGARISCAPPIO, F ;
BERGUI, L ;
TESIO, L ;
PIZZOLO, G ;
MALAVASI, F ;
CHILOSI, M ;
CAMPANA, D ;
VANCAMP, B ;
JANOSSY, G .
JOURNAL OF CLINICAL INVESTIGATION, 1985, 76 (03) :1243-1251
[3]  
DILOSA RM, 1991, J IMMUNOL, V146, P4071
[4]  
HARADA H, 1993, BLOOD, V81, P2658
[5]  
HARADA N, 1993, J IMMUNOL, V151, P3111
[6]   GROWTH-FACTOR REQUIREMENTS FOR THE STIMULATION OF GERMINAL CENTER B-CELLS - EVIDENCE FOR AN IL-2-DEPENDENT PATHWAY OF DEVELOPMENT [J].
HOLDER, MJ ;
LIU, YJ ;
DEFRANCE, T ;
FLORESROMO, L ;
MACLENNAN, ICM ;
GORDON, J .
INTERNATIONAL IMMUNOLOGY, 1991, 3 (12) :1243-1251
[7]   FORMATION AND HYDROLYSIS OF CYCLIC ADP RIBOSE CATALYZED BY LYMPHOCYTE ANTIGEN-CD38 [J].
HOWARD, M ;
GRIMALDI, JC ;
BAZAN, JF ;
LUND, FE ;
SANTOSARGUMEDO, L ;
PARKHOUSE, RME ;
WALSETH, TF ;
LEE, HC .
SCIENCE, 1993, 262 (5136) :1056-1059
[8]  
HUANG NH, 1993, BLOOD, V82, P3721
[9]   CYCLIC ADP RIBOSE IN BETA-CELLS [J].
ISLAM, MS ;
LARSSON, O ;
BERGGREN, PO .
SCIENCE, 1993, 262 (5133) :584-585
[10]  
IWATO K, 1988, BLOOD, V72, P562