Nicotiana benthamiana plants expressing beet necrotic yellow vein virus (BNYVV) coat protein-specific scFv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection

被引：60

作者：

Fecker, LF ^{[1
]}

Koenig, R ^{[1
]}

Obermeier, C ^{[1
]}

机构：

[1] BIOL BUNDESANSTALT LAND & FORSTWIRTSCHAFT,INST BIOCHEM & PFLANZENVIROL,D-38104 BRAUNSCHWEIG,GERMANY

来源：

ARCHIVES OF VIROLOGY | 1997年 / 142卷 / 09期

关键词：

D O I：

10.1007/s007050050203

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Transgenic plants of Nicotiana benthamiana expressing single chain antibody fragments (scFv) specific for the coat protein of beet necrotic yellow vein virus (BNYVV) and non-expressing control plants were inoculated with BNYVV mechanically and by means of the vector Polymyxa betae. The scFv were presumably expressed in the endoplasmic reticulum (ER). The average time needed for infections to become detectable was longer in the scFv-expressing plants than in the non-expressing control plants. In addition, the scFv-expressing plants were partially protected against the pathogenic effects exerted by the virus on N. benthamiana plants in the late stages of infection.

引用

页码：1857 / 1863

页数：7

共 19 条

[1] A SINGLE NUCLEOTIDE CHANGE IN THE COAT PROTEIN GENE OF TOBACCO MOSAIC-VIRUS IS INVOLVED IN THE INDUCTION OF SEVERE CHLOROSIS [J].