Molecular cloning and characterization of two novel transport proteins from rat kidney

被引：81

作者：

Schömig, E ^{[1
]}

Spitzenberger, F ^{[1
]}

Engelhardt, M ^{[1
]}

Martel, F ^{[1
]}

Örding, N ^{[1
]}

Gründemann, D ^{[1
]}

机构：

[1] Univ Heidelberg, Dept Pharmacol, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 1998年 / 425卷 / 01期

关键词：

organic cation transport; organic anion transport; amphiphilic solute facilitator family; phylogeny; evolution;

D O I：

10.1016/S0014-5793(98)00203-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent cloning of renal transport systems for organic anions and cations (OAT1, OCT1, and OCT2) opened the possibility to search, via polymerase chain reaction (PCR) homology screening, for novel transport proteins. Two integral membrane proteins, UST1 and UST2, were cloned from rat kidney. RT-PCR revealed that UST1 is confined to the kidney whereas UST2 mRNA was detected in all tested tissues. Sequence analyses suggest that UST1 and UST2, together with four related transporters, comprise, within the major facilitator superfamily, a so far unrecognized transporter family, termed amphiphilic solute facilitator (ASF) family. Characteristic signatures for the ASF family were identified. (C) 1998 Federation of European Biochemical Societies.

引用

页码：79 / 86

页数：8

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