Wogonin Inhibits Osteoclast Formation Induced by Lipopolysaccharide

被引：8

作者：

Jang, Sungil ^{[1
,2
,3
]}

Bak, Eun Jung ^{[1
,2
]}

Kim, Minyoung ^{[1
,2
,3
]}

Kim, Jin Moon ^{[1
,2
,3
]}

Chung, Won-Yoon ^{[1
,2
,3
]}

Cha, Jeong-Heon ^{[1
,2
,3
]}

Yoo, Yun-Jung ^{[1
,2
,3
]}

机构：

[1] Yonsei Univ, Coll Dent, Dept Oral Biol, Oral Sci Res Ctr,Project BK21, Seoul 120752, South Korea

[2] Yonsei Univ, Coll Dent, Res Ctr Orofacial Hard Tissue Regenerat, Seoul 120752, South Korea

[3] Yonsei Univ, Grad Sch, Dept Appl Life Sci, Seoul 120749, South Korea

来源：

PHYTOTHERAPY RESEARCH | 2010年 / 24卷 / 07期

关键词：

wogonin; osteoclastogenesis; lipopolysaccharide; NITRIC-OXIDE SYNTHASE; PROSTAGLANDIN E-2; BONE LOSS; PLANT FLAVONE; DIFFERENTIATION; SUPPRESSION; CYCLOOXYGENASE-2; OSTEOPROTEGERIN; INTERLEUKIN-6; EXPRESSION;

D O I：

10.1002/ptr.3013

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

To evaluate the inhibitory activity of wogonin against lipopolysaccharide (LPS)-induced bone resorption, we investigated the effect of wogonin on osteoclastogenesis induced by LPS. Wogonin inhibited LPS-induced osteoclastogenesis in co-cultures of mouse calvaria-derived osteoblasts and bone marrow-derived pre-osteoclasts. Wogonin also suppressed osteoclastogenesis in LPS-injected mouse calvaria. In osteoblasts, the upregulation of receptor activator of nuclear factor-kappa B (RANKL) expression and the downregulation of osteoprotegerin (OPG) expression by LPS were inhibited by wogonin. Wogonin and NS-398, a COX-2 inhibitor, suppressed LPS-stimulated PGE(2) production in osteoblasts. NS-398 inhibited the effect of LPS on RANKL and OPG expression in osteoblasts. These results suggest that wogonin acts as an inhibitor of LPS-induced osteoclastogenesis through downregulation of RANKL and upregulation of OPG expression via blockage of PGE(2) production. Based on these results, wogonin has potential for use as a therapeutic agent in bacteria-induced bone resorption. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：964 / 968

页数：5

共 22 条

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