A molecular genetic approach for species identification of mammals and sex determination of birds in a forensic case of poaching from South Korea

被引:34
作者
An, Junghwa [1 ]
Lee, Mu-yeong [1 ]
Min, Mi-Sook [1 ]
Lee, Mun-Han [1 ]
Lee, Hang [1 ]
机构
[1] Seoul Natl Univ, Coll Vet Med, Sch Agr Biotechnol, CGRB Korean Wildlife, Seoul 151742, South Korea
关键词
species identification; cytochrome b gene; sex determination; CHD gene;
D O I
10.1016/j.forsciint.2005.12.031
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
DNA-based analysis was performed using partial mitochondrial cytochrome b genes of five mammalian specimens and Chromo-Helicase-DNA-binding (CHD) genes of five pheasants to determine whether specimens were from illegally hunted animals. Mammalian specimens were identified as being those of horse, roe deer, and cow through gene amplification using cytb981f and cytb981r primer set and sequencing. CHD genes were revealed to be those of three male and two female pheasants through polymerase chain reaction amplification. Because hunting of roe deers and female pheasants is prohibited in Korea, these results provided forensic evidences of illegal wild animal hunting. (C) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:59 / 61
页数:3
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