Immunocytochemical localization of nicotinic acetylcholine receptor in the rat cerebellar cortex

被引:21
作者
Nakayama, H
Shioda, S
Nakajo, S
Ueno, S
Nakashima, T
Nakai, Y
机构
[1] Nara Med Univ, Dept Pharmacol, Kashihara, Nara 634, Japan
[2] Showa Univ, Sch Med, Dept Anat, Tokyo 142, Japan
[3] Showa Univ, Sch Pharmaceut Sci, Biol Chem Lab, Tokyo 142, Japan
[4] Nara Med Univ, Dept Med Genet, Kashihara, Nara 634, Japan
关键词
acetylcholine receptor; alpha; 4; subunit; cerebellum; immunocytochemistry; nicotinic; presynapse; postsynapse; rat brain;
D O I
10.1016/S0168-0102(97)00100-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Although nicotinic acetylcholine receptors (nAChRs) have been reported to function in the cerebellar cortex, nicotinic acetylcholine receptor subtypes contributing to nicotinic currents in the cortex have not been well characterized at the subcellular level. This study deals with immunocytochemical localization of the nicotinic acetylcholine receptor alpha 4 subunit using a monoclonal antibody against the alpha 4 subunit. alpha L-LI (alpha 4-like immunoreactivity) was detected in the cell bodies of molecular, Purkinje cell and granular layers. In particular, the cell bodies of Purkinje cells were extensively immunostained. In Purkinje cells, alpha 4-LI was found in perikarya mainly associated with rough endoplasmic reticulum, plasma membrane, and cytoplasmic matrix. At higher magnification, the immunoreaction product was densely localized along with somatic plasma membranes at the axe-somatic synapse and the plasma membranes at extrasynaptic regions of cell bodies. alpha 4-LI was also found in the axon terminals which form synapses with Purkinje cells. In the granular layer, somatic cell membranes of granular cells were immunostained. These morphological observations suggest that alpha 4-containing nAChRs contribute nicotinic currents reported in Purkinje cells, and that presynaptic alpha 4-containing nAChRs regulate the release of neurotransmitters on the axon terminals found near Purkinje cells. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:233 / 239
页数:7
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