Preconditioning prevents the inhibition of Na+,K+-ATPase activity after brain ischemia

被引：159

作者：

Wyse, ATD ^{[1
]}

Streck, EL ^{[1
]}

Worm, P ^{[1
]}

Wajner, A ^{[1
]}

Ritter, F ^{[1
]}

Netto, CA ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil

来源：

NEUROCHEMICAL RESEARCH | 2000年 / 25卷 / 07期

关键词：

Na+; K+-ATPase; brain ischemia; ischemic preconditioning;

D O I：

10.1023/A:1007504525301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Application of single transient forebrain ischemia (ISC) in adult Wistar rats, lasting 2 or 10 min, caused inhibition of Na+,K+-ATPase activity in cytoplasmic membrane fractions of hippocampus and cerebral cortex immediately after the event. In the 2-min ISC group followed by 60 min of reperfusion, the enzyme inhibition was maintained in the cortex, while there was an increase in hippocampal enzyme activity; both effects were over 1 day after the event. However, in the 10-min ISC group enzyme inhibition had been maintained for 7 days in both cerebral structures. Interestingly, ischemic preconditioning (2-min plus l0-min ISC, with a 24-hour interval in between) prevented the inhibitory effect of ischemia/reperfusion on Na+,K+-ATPase activity observed either after a single insult of 2 min or 10 min ischemia. We suggest that the maintenance of Na+,K+-ATPase activity afforded by preconditioning be related to cellular neuroprotection.

引用

页码：971 / 975

页数：5

共 32 条

[1]

BANERJEE A, 1993, CIRC RES, V28, P1726

[2]

Bertorello AM, 1995, NEWS PHYSIOL SCI, V10, P253

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] INHIBITION OF ALPHA-2/ALPHA-3 SODIUM-PUMP ISOFORMS POTENTIATES GLUTAMATE NEUROTOXICITY [J].