Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

被引：51

作者：

Chong, PP

Podmore, SM

Kieser, HM

Redenbach, M

Turgay, K

Marahiel, M

Hopwood, DA

Smith, CP

机构：

[1] Univ Manchester, Inst Sci & Technol, Dept Biochem & Appl Mol Biol, Manchester M60 1QD, Lancs, England

[2] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

[3] Univ Kaiserslautern, Genome Res Unit, Dept Genet, D-67663 Kaiserslautern, Germany

[4] Univ Marburg, FB Chem, D-35032 Marburg, Germany

来源：

MICROBIOLOGY-UK | 1998年 / 144卷

关键词：

Streptomyces coelicolor; antibiotic biosynthesis; lipopeptide; gene disruption; peptide synthetase;

D O I：

10.1099/00221287-144-1-193

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single similar to 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA), and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.

引用

页码：193 / 199

页数：7

共 33 条

[1] The regulation of antibiotic production in Streptomyces coelicolor A3(2) [J].