Coronary vasoconstriction produced by vasopressin in anesthetized goats.: Role of vasopressin V1 and V2 receptors and nitric oxide

被引:28
作者
Fernández, N [1 ]
García, JL [1 ]
García-Villalón, AL [1 ]
Monge, L [1 ]
Gómez, B [1 ]
Diéguez, G [1 ]
机构
[1] Univ Autonoma Madrid, Fac Med, Dept Fisiol, E-28029 Madrid, Spain
关键词
coronary circulation; coronary blood flow; vasopressin receptor; N-omega-nitro-L-arginine methyl ester (L-NAME); prostanoid;
D O I
10.1016/S0014-2999(97)01504-5
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
To examine the role of vasopressin V-1 and V-2 receptors, nitric oxide and prostanoids in the coronary vascular effects of [Arg(8)]vasopressin, coronary blood flow was measured with an electromagnetic flow transducer placed around the left circumflex (23 goats) or anterior descending(ll goats) coronary artery and vasopressin (0.03-1 mu g) was intracoronarily injected in 34 anesthetized, open-chest goats. Basal mean values for coronary blood flow, mean systemic arterial pressure and heart rate, were 34 +/- 2.38 ml/min, 89 +/- 3.34 mmHg and 80 +/- 3.06 beats/min, respectively. Vasopressin produced dose-dependent decreases in coronary blood flow and the maximal reduction of this flow, attained with 1 mu g of vasopressin, was 14 +/- 1.49 ml/min (42 +/- 2.64% of basal flow) (P < 0.01). Desmopressin (0.03-1 mu g; 8 goats) did not affect significantly coronary blood flow. The intracoronary infusion of the antagonist for vasopressin V-1 receptors d(CH2)(5)Tyr (Me) arginine vasopressin (2 mu g/min per kg, 6 animals) significantly diminished the effects of vasopressin on coronary blood flow (the effects of 1 mu g of vasopressin were reduced by 28%, P < 0.05). The mixed antagonist for vasopressin V-1 and V-2 receptors desGly-d(CH2)(5)-D-Tyr(Et)Val arginine vasopressin(0.2, 0.7 and 2 mu g/min per kg, 9 animals) decreased in a dose-dependent manner the effects of vasopressin on coronary blood flow (the effects of 1 mu g of vasopressin were decreased by 61% with 2 mu g/min per kg, P < 0.01). Intracoronary infusion of saline (vehicle, 3 goats) did not change the effects of vasopressin on coronary blood flow. Intravenous administration of the inhibitor of nitric oxide synthesis N-w-nitro-L-arginine methyl ester (L-NAME, 47 mg/kg, 9 animals) decreased resting coronary blood flow by 10% (P < 0.01) and augmented mean systemic arterial pressure by 20% (P < 0.01), without changing heart rate. During this treatment the reduction in coronary blood flow produced by vasopressin was higher than under control (the effects of 1 mu g of vasopressin were increased by 28%, P<0.01). Intravenous administration of the inhibitor of cyclooxygenase, meclofenamate (5 mg/kg, 7 animals), neither modified resting coronary blood flow, arterial pressure and heart rate nor the effects of vasopressin on this flow. These data indicate that vasopressin produces marked coronary vasoconstriction and suggest that: (a) it may be mediated by vasopressin V-1 receptors, without involvement of vasopressin V-2 receptors, (b) it is probably inhibited by nitric oxide under normal conditions and (c) it may be not modulated by prostanoids. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:225 / 233
页数:9
相关论文
共 35 条
[1]  
ABBOUD FM, 1990, BLOOD VESSELS, V27, P106
[2]  
ALTURA BM, 1984, FED PROC, V43, P80
[4]   [ARG(8)]VASOPRESSIN-INDUCED RESPONSES OF THE HUMAN ISOLATED CORONARY-ARTERY - EFFECTS OF NONPEPTIDE RECEPTOR ANTAGONISTS [J].
BAX, WA ;
VANDERGRAAF, PH ;
STAM, WB ;
BOS, E ;
NISATO, D ;
SAXENA, PR .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1995, 285 (02) :199-202
[5]  
BERDE B, 1965, ADV OXYTOCIN RESEARC, P11
[6]   EFFECTS OF INHIBITION OF NITRIC-OXIDE FORMATION ON BASAL VASOMOTION AND ENDOTHELIUM-DEPENDENT RESPONSES OF THE CORONARY-ARTERIES IN AWAKE DOGS [J].
CHU, A ;
CHAMBERS, DE ;
LIN, CC ;
KUEHL, WD ;
PALMER, RMJ ;
MONCADA, S ;
COBB, FR .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (06) :1964-1968
[7]   SYSTEMIC AND CORONARY HEMODYNAMIC EFFECTS OF VASOPRESSIN [J].
CORLISS, RJ ;
MCKENNA, DH ;
SIALER, S ;
OBRIEN, GS ;
ROWE, GG .
AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 1968, 256 (05) :293-+
[8]  
Cowley AW, 1987, Vasopressin: Principles and Properties, P389
[9]   PLASMA CORTICOTROPIN-RELEASING HORMONE, VASOPRESSIN, ACTH AND CORTISOL RESPONSES TO ACUTE MYOCARDIAL-INFARCTION [J].
DONALD, RA ;
CROZIER, IG ;
FOY, SG ;
RICHARDS, AM ;
LIVESEY, JH ;
ELLIS, MJ ;
MATTIOLI, L ;
IKRAM, H .
CLINICAL ENDOCRINOLOGY, 1994, 40 (04) :499-504
[10]   EFFECTS OF NITRIC-OXIDE SYNTHESIS INHIBITION ON THE GOAT CORONARY CIRCULATION UNDER BASAL CONDITIONS AND AFTER VASODILATOR STIMULATION [J].
GARCIA, JL ;
FERNANDEZ, N ;
GARCIAVILLALON, AL ;
MONGE, L ;
GOMEZ, B ;
DIEGUEZ, G .
BRITISH JOURNAL OF PHARMACOLOGY, 1992, 106 (03) :563-567