Glucocorticoid mediated transcriptional repression of c-myc in apoptotic human leukemic CEM cells

被引：28

作者：

Zhou, F ^{[1
]}

Medh, RD ^{[1
]}

Thompson, EB ^{[1
]}

机构：

[1] Univ Texas, Med Branch, Dept Human Biol Chem & Genet, Galveston, TX 77555 USA

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2000年 / 73卷 / 05期

关键词：

glucocorticoids; c-myc; apoptosis; lymphoid; CEM cells;

D O I：

10.1016/S0960-0760(00)00080-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Suppression of c-myc has been implicated as a critical event in some glucocorticoid-evoked apoptotic systems. It is therefore of interest to understand the mechanism of glucocorticoid-regulation of the c-myc gene. In the present study, a detailed analysis of dexamethasone (Dex)-evoked regulation of the human c-myc gene in human leukemic CEM-C7 cells has been performed. Dex suppresses c-myc mRNA and immunoreactive protein expression in clone CEM-C7 and subclone CEM-C7-14 cells. Nuclear run-on assays suggested that the regulation occurred at the level of transcription initiation. The half-life of c-myc mRNA was approximately 30 min and its stability was not affected by Dex treatment. In addition, Dex suppressed luciferase gene expression driven by - 2052 to + 34 bp c-myc promoter in transfected CEM-C7-14 cells. This result further supports that c-myc gene is suppressed by Dex at the transcriptional level in apoptotic human leukemic cells. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：195 / 202

页数：8

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