Heat shock proteins and inflammatory acne vulgaris:: molecular cloning, overexpression and purification of a Propionibacterium acnes GroEL and DnaK homologue

被引：16

作者：

Farrar, MD ^{[1
]}

Ingham, E ^{[1
]}

Holland, KT ^{[1
]}

机构：

[1] Univ Leeds, Sch Biochem & Mol Biol, Div Microbiol, Skin Res Ctr, Leeds LS2 9JT, W Yorkshire, England

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 191卷 / 02期

基金：

英国医学研究理事会;

关键词：

acne vulgaris; Propionibacterium acnes; GroEL; DnaK;

D O I：

10.1016/S0378-1097(00)00387-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Propionibacterium acnes is associated with inflammatory acne. The genes encoding two putative mediators of inflammation, the heat shock proteins GroEL and DnaK, were cloned from this organism and sequenced. groEL and dnaK encode proteins of 56.8 and 66.4 kDa, respectively, which show a high degree of homology (>75% similarity) to the GroEL and DnaK proteins of mycobacteria and streptomycetes. The promoter regions of both genes contain inverted repeat sequences believed to be involved in the transcriptional regulation of heat shock genes. Recombinant P. acnes GroEL and DnaK were overexpressed in Escherichia coli with C-terminal histidine tags. The recombinant proteins were purified from E. coli by metal affinity chromatography. These proteins will now be used in immunological investigations to determine their role in inflammatory acne. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：183 / 186

页数：4

共 18 条

[1]

Regulation of the dnaK operon of Streptomyces coelicolor A3(2) is governed by HspR, an autoregulatory repressor protein [J].