Glucose-Regulated Protein 78 (GRP78) Silencing Enhances Cell Migration but Does Not Influence Cell Proliferation in Hepatocellular Carcinoma

被引：40

作者：

Chang, Yu-Jia ^{[1
,2
,3
]}

Chiu, Chong-Chi ^{[4
]}

Wu, Chih-Hsiung ^{[1
,5
]}

An, Jane ^{[6
]}

Wu, Cheng-Chia ^{[6
]}

Liu, Tsan-Zon ^{[2
,3
]}

Wei, Po-Li ^{[1
,2
,3
]}

Huang, Ming-Te ^{[1
,5
]}

机构：

[1] Taipei Med Univ, Dept Surg, Taipei, Taiwan

[2] Taipei Med Univ, Taipei Med Univ Hosp, Translat Res Lab Canc Ctr, Taipei, Taiwan

[3] Taipei Med Univ, Taipei Med Univ Hosp, Dept Surg, Taipei, Taiwan

[4] Chi Mei Med Ctr, Dept Surg, Tainan, Taiwan

[5] Taipei Med Univ, Shuang Ho Hosp, Dept Surg, Taipei, Taiwan

[6] New York Med Coll, Valhalla, NY 10595 USA

来源：

ANNALS OF SURGICAL ONCOLOGY | 2010年 / 17卷 / 06期

关键词：

CANCER-CELLS; PROGNOSTIC-FACTORS; INDUCED APOPTOSIS; ACTIVATION; INDUCTION; RESECTION; GROWTH; OVEREXPRESSION; METASTASIS; INHIBITION;

D O I：

10.1245/s10434-010-0912-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

GRP78 plays an essential role in embryonic development and in the therapeutic treatment and progression of cancer. However, little is known about the role of GRP78 in hepatocellular carcinoma (HCC). In this study, we characterized five different HCC cell lines to examine GRP78 expression patterns and found that only HepJ5 cells ectopically overexpress GRP78. We knocked down GRP78 expression in HepJ5 cells using a small interfering RNA (siRNA), and the proliferation assay and migration assay were performed. Using siRNA technique, we could successfully reduce GRP78 expression levels in HepJ5 cells. In a cell growth study, we found that GRP78-siRNA caused no significant changes in cellular proliferation in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, and cell cycle distribution. In a cell migration study, we found that GRP78-siRNA HepJ5 cells had dramatically increased migration ability in Transwell assay. We conclude that ectopically expressed GRP78 does not contribute to the increased proliferation of HepJ5 cells, but does correlate with the migration of HCC cells under normoxic conditions.

引用

页码：1703 / 1709

页数：7

共 47 条

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