Identification of SCAR markers linked to PI-w mildew resistance in apple

被引:53
作者
Evans, KM [1 ]
James, CM [1 ]
机构
[1] Hort Res Int, E Malling ME19 6BJ, Kent, England
关键词
apple; resistance; powdery mildew; molecular marker; SCAR;
D O I
10.1007/s00122-002-1147-2
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Resistance to powdery mildew is an important objective for cultivar improvement programmes of apple and several different major genes for resistance to mildew are available. Molecular markers linked to such key traits can be used to screen progenies for resistant individuals. A progeny derived from the crab apple 'White Angel' (the source of Pl-w) was screened for resistance to mildew for two seasons in the glasshouse and four seasons in the field. DNA bulks of resistant and susceptible seedlings were screened with 176 AFLP primer combinations. Seven AFLP markers were identified that differentiated the bulks, and two of these markers were developed into SCARs, EM M01 and EM M02, mapping at 4.6 and 6.4 recombination units from Pl-w.
引用
收藏
页码:1178 / 1183
页数:6
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