Interaction of adrenomedullin with calcitonin receptor in cultured human breast cancer cells, T 47D

被引：16

作者：

Disa, J

Dang, K

Tan, KB

Aiyar, N

机构：

[1] SmithKline Beecham Pharmaceut, Dept Cardiovasc Pharmacol, King Of Prussia, PA 19406 USA

[2] SmithKline Beecham Pharmaceut, Dept Mol Immunol, King Of Prussia, PA 19406 USA

来源：

PEPTIDES | 1998年 / 19卷 / 02期

关键词：

adrenomedullin; salmon calcitonin; antagonist; adenylyl cyclase; receptors;

D O I：

10.1016/S0196-9781(97)00376-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human adrenomedullin (hADM), human calcitonin gene-related peptide (hCGRP), and salmon calcitonin (sCT)-activated adenylyl cyclase with EC50, values of 132, 764, and 0.5 nM, respectively, in human breast cancer cell line, T 47D. Treatment of T 47D cell membranes with near maximal concentrations of sCT, hADM and hCGRP had no additive effect on adenylyl cyclase activity. Salmon calcitonin (8-32)(sCT (8-32)), selective antagonist of calcitonin receptor, inhibited the activation of adenylyl cyclase by these three peptides. On the other hand, the putative ADM receptor antagonist, ADM (22-52), and CGRP receptor antagonist, CGRP (8-37), failed to inhibit ADM-, CGRP- or sCT-activated adenylyl cyclase. These results suggest that in T47D cells, both ADM and CGRP activated adenylyl cyclase through sCT receptors. (C) 1998 Elsevier Science Inc.

引用

页码：247 / 251

页数：5

共 15 条

[1] IDENTIFICATION AND CHARACTERIZATION OF CALCITONIN GENE-RELATED PEPTIDE RECEPTORS IN PORCINE RENAL MEDULLARY MEMBRANES [J].