Immunomorphological Pattern of Molecular Chaperones in Normal and Pathological Thyroid Tissues and Circulating Exosomes: Potential Use in Clinics

被引：43

作者：

Bavisotto, Celeste Caruso ^{[1
,2
,3
]}

Cipolla, Calogero ^{[4
]}

Graceffa, Giuseppa ^{[4
]}

Barone, Rosario ^{[1
]}

Bucchieri, Fabio ^{[1
,5
]}

Bulone, Donatella ^{[3
]}

Cabibi, Daniela ^{[6
]}

Campanella, Claudia ^{[1
]}

Gammazza, Antonella Marino ^{[1
]}

Pitruzzella, Alessandro ^{[1
]}

Porcasi, Rossana ^{[6
]}

San Biagio, Pier Luigi ^{[3
]}

Tomasello, Giovanni ^{[1
]}

de Macario, Everly Conway ^{[2
,7
]}

Macario, Alberto J. L. ^{[2
,7
]}

Cappello, Francesco ^{[1
,2
]}

Rappa, Francesca ^{[1
]}

机构：

[1] Univ Palermo, Dept Biomed Neurosci & Adv Diagnost BIND, Inst Human Anat & Histol, I-90127 Palermo, Italy

[2] Euromediterranean Inst Sci & Technol IEMEST, I-90100 Palermo, Italy

[3] CNR, Inst Biophys, I-90100 Palermo, Italy

[4] Univ Palermo, Dept Surg Oncol & Oral Sci, I-90127 Palermo, Italy

[5] CNR, Inst Biomed & Mol Immunol, I-90100 Palermo, Italy

[6] Univ Palermo, Pathol Inst, Dept G DAlessandro, I-90127 Palermo, Italy

[7] Univ Maryland, IMET, Sch Med, Dept Microbiol & Immunol, Baltimore, MD 21202 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 18期

关键词：

thyroid gland; papillary carcinoma; molecular chaperones; heat shock proteins (Hsp); goiter; exosomes; diagnosis; HEAT-SHOCK PROTEINS; CELL-DEATH; CANCER; HSP27; CARCINOMA; BIOGENESIS; ACTIVATION; EXPRESSION; APOPTOSIS; COMPLEX;

D O I：

10.3390/ijms20184496

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The thyroid is a major component of the endocrine system and its pathology can cause serious diseases, e.g., papillary carcinoma (PC). However, the carcinogenic mechanisms are poorly understood and clinical useful biomarkers are scarce. Therefore, we determined if there are quantitative patterns of molecular chaperones in the tumor tissue and circulating exosomes that may be useful in diagnosis and provide clues on their participation in carcinogenesis. Hsp27, Hsp60, Hsp70, and Hsp90 were quantified by immunohistochemistry in PC, benign goiter (BG), and normal peritumoral tissue (PT). The same chaperones were assessed in plasma exosomes from PC and BG patients before and after ablative surgery, using Western blotting. Hsp27, Hsp60, and Hsp90 were increased in PC in comparison with PT and BG but no differences were found for Hsp70. Similarly, exosomal levels of Hsp27, Hsp60, and Hsp90 were higher in PC than in BG, and those in PC were higher before ablative surgery than after it. Hsp27, Hsp60, and Hsp90 show distinctive quantitative patterns in thyroid tissue and circulating exosomes in PC as compared with BG, suggesting some implication in the carcinogenesis of these chaperones and indicating their potential as biomarkers for clinical applications.

引用

页数：12

共 42 条

[1]

Exosomal HSP60: a potentially useful biomarker for diagnosis, assessing prognosis, and monitoring response to treatment [J].

Bavisotto, Celeste Caruso ;

Cappello, Francesco ;

Macario, Alberto J. L. ;

de Macario, Everly Conway ;

Logozzi, Mariantonia ;

Fais, Stefano ;

Campanella, Claudia .

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2017, 17 (09) :815-822

[2]

The dissociation of the Hsp60/pro-Caspase-3 complex by bis(pyridyl)oxadiazole copper complex (CubipyOXA) leads to cell death in NCI-H292 cancer cells [J].

Bavisotto, Celeste Caruso ;

Nikolic, Dragana ;

Gammazza, Antonella Marino ;

Barone, Rosario ;

Lo Cascio, Filippa ;

Mocciaro, Emanuele ;

Zummo, Giovanni ;

de Macario, Everly Conway ;

Macario, Alberto J. L. ;

Cappello, Francesco ;

Giacalone, Valentina ;

Pace, Andrea ;

Barone, Giampaolo ;

Piccionello, Antonio Palumbo ;

Campanella, Claudia .

JOURNAL OF INORGANIC BIOCHEMISTRY, 2017, 170 :8-16

[3]

Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome [J].

Beere, HM ;

Wolf, BB ;

Cain, K ;

Mosser, DD ;

Mahboubi, A ;

Kuwana, T ;

Tailor, P ;

Morimoto, RI ;

Cohen, GM ;

Green, DR .

NATURE CELL BIOLOGY, 2000, 2 (08) :469-475

[4]

Hsp27 negatively regulates cell death by interacting with cytochrome c [J].

Bruey, JM ;

Ducasse, C ;

Bonniaud, P ;

Ravagnan, L ;

Susin, SA ;

Diaz-Latoud, C ;

Gurbuxani, S ;

Arrigo, AP ;

Kroemer, G ;

Solary, E ;

Garrido, C .

NATURE CELL BIOLOGY, 2000, 2 (09) :645-652

[5]

Campanella C, 2008, EUR J HISTOCHEM, V52, P221

[6]

Campanella C., 2014, J. Circ. Biomarkers, V3, P1, DOI [10.5772/58721, DOI 10.5772/58721]

[7]

On the Choice of the Extracellular Vesicles for Therapeutic Purposes [J].

Campanella, Claudia ;

Bavisotto, Celeste Caruso ;

Logozzi, Mariantonia ;

Gammazza, Antonella Marino ;

Mizzoni, Davide ;

Cappello, Francesco ;

Fais, Stefano .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (02)

[8]

The histone deacetylase inhibitor SAHA induces HSP60 nitration and its extracellular release by exosomal vesicles in human lung-derived carcinoma cells [J].

Campanella, Claudia ;

D'Anneo, Antonella ;

Gammazza, Antonella Marino ;

Bavisotto, Celeste Caruso ;

Barone, Rosario ;

Emanuele, Sonia ;

Lo Cascio, Filippa ;

Mocciaro, Emanuele ;

Fais, Stefano ;

De Macario, Everly Conway ;

Macario, Alberto J. L. ;

Cappello, Francesco ;

Lauricella, Marianna .

ONCOTARGET, 2016, 7 (20) :28849-28867

[9]

Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery [J].

Campanella, Claudia ;

Rappa, Francesca ;

Sciume, Carmelo ;

Marino Gammazza, Antonella ;

Barone, Rosario ;

Bucchieri, Fabio ;

David, Sabrina ;

Curcuru, Giuseppe ;

Caruso Bavisotto, Celeste ;

Pitruzzella, Alessandro ;

Geraci, Girolamo ;

Modica, Giuseppe ;

Farina, Felicia ;

Zummo, Giovanni ;

Fais, Stefano ;

de Macario, Everly Conway ;

Macario, Alberto J. L. ;

Cappello, Francesco .

CANCER, 2015, 121 (18) :3230-3239

[10]

The Odyssey of Hsp60 from Tumor Cells to Other Destinations Includes Plasma Membrane-Associated Stages and Golgi and Exosomal Protein-Trafficking Modalities [J].

Campanella, Claudia ;

Bucchieri, Fabio ;

Merendino, Anna M. ;

Fucarino, Alberto ;

Burgio, Giosalba ;

Corona, Davide F. V. ;

Barbieri, Giovanna ;

David, Sabrina ;

Farina, Felicia ;

Zummo, Giovanni ;

de Macario, Everly Conway ;

Macario, Alberto J. L. ;

Cappello, Francesco .

PLOS ONE, 2012, 7 (07)

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