Sleeve gastrectomy drives persistent shifts in the gut microbiome

被引：46

作者：

Jahansouz, Cyrus ^{[1
]}

Staley, Christopher ^{[2
]}

Bernlohr, David A. ^{[3
]}

Sadowsky, Michael J. ^{[2
]}

Khoruts, Alexander ^{[2
,4
,5
]}

Ikramuddin, Sayeed ^{[1
]}

机构：

[1] Univ Minnesota, Dept Surg, 420 Delaware St SE, Minneapolis, MN 55455 USA

[2] Univ Minnesota, BioTechnol Inst, St Paul, MN 55108 USA

[3] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

[4] Univ Minnesota, Div Gastroenterol, Dept Med, Minneapolis, MN 55455 USA

[5] Univ Minnesota, Ctr Immunol, Minneapolis, MN 55455 USA

来源：

SURGERY FOR OBESITY AND RELATED DISEASES | 2017年 / 13卷 / 06期

关键词：

Microbiome; Obesity; OBESITY; ADAPTATION; SURGERIES; RICHNESS;

D O I：

10.1016/j.soard.2017.01.003

中图分类号：

R61 [外科手术学];

学科分类号：

100210 [外科学];

摘要：

Background: Changes in the gut microbiome following bariatric surgery have been causally linked to metabolic benefits. Objectives: We sought to characterize and assess the stability of gut microbiome shifts following sleeve gastrectomy (SG). Setting: University laboratories. Methods: Diet-induced obese mice were randomized to SG or sham surgery. Mice were housed individually or cohoused such that one SG mouse was housed with one weight-matched, sham operated mouse. Fecal samples were collected before and on postoperative days 7 and 28. Bacterial composition in feces was characterized by using next-generation Illumina sequencing of 16 S rRNA. Results: SG mice lost more weight and were more insulin sensitive than sham mice independent of housing status (P<.05). One week following surgery, fecal samples from all mice showed shifts in the microbiome that only persisted in SG-operated mice. Cohousing did not alter the microbial composition of SG-operated mice. Cohoused sham-operated mice showed a unique shift in microbial composition on postoperative day 28 that differed from individually housed, sham-operated mice (P<.001). Cohousing did not affect metabolic outcomes of either SG or sham surgeries. Conclusion: SG results in acute and sustained shifts in the gut microbiome. SG associated shifts are not altered by reexposure to obesity-associated gut microbiota. (C) 2017 American Society for Metabolic and Bariatric Surgery. All rights reserved.

引用

页码：916 / 924

页数：9

共 36 条

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