Agrobacterium-mediated transformation of African violet

被引：24

作者：

Mercuri, A ^{[1
]}

De Benedetti, L ^{[1
]}

Burchi, G ^{[1
]}

Schiva, T ^{[1
]}

机构：

[1] Ist Sperimentale Floricultura, I-18038 San Remo, IM, Italy

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2000年 / 60卷 / 01期

关键词：

African violet; GUS; nptII; PCR; Saintpaulia ionantha;

D O I：

10.1023/A:1006457716959

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method for genetic transformation of Saintpaulia ionantha by co-cultivation of in vitro-grown leaves and petioles with Agrobacterium tumefaciens is described. Two bacterial strains, EHA105 and A281 both harbouring the binary plasmid pKIWI105 carrying the genes uidA and nptII, were used in the experiments. Regenerants were not obtained using the disarmed strain EHA105. The oncogenic strain A281 resulted in efficient transient and stable expression of the transferred traits for petiole explants only. After transformation and regeneration, the integration of the transgenes in the plant genome was confirmed by PCR analysis and Southern hybridization.

引用

页码：39 / 46

页数：8

共 22 条

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