Agrobacterium-mediated transformation of Asarina procumbens Mill.

被引:2
作者
Cui, ML
Ichikawa, H
Sage-Ono, K
Kamada, H
Handa, T [1 ]
机构
[1] Univ Tsukuba, Inst Agr & Forestry, Tsukuba, Ibaraki 3058572, Japan
[2] Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan
[3] Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan
关键词
D O I
10.1080/14620316.2003.11511637
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
This paper reports on protocols for plant regeneration and transformation of Asarina procumbens Mill. (syn. Antirrhinum asarina L.). Asarina is an ornamental plant, in this study we successfully achieved shoot regeneration from stem explants on MS medium supplemented with zeatin. Furthermore, the transformation system was established via Agrobacterium-mediated transformation with stem segments. The A. tumefaciens strains EHA105, GV2260 and GV3101 each harbouring binary vector pSMAK251 containing uidA and npt II genes were used in this establishment. Kanamycin-resistant shoots regenerated directly on the selection medium containing 2 mg 1(-1) zeatin, 100 mg 1(-1) kanamycin, and 250 mg 1(-1) cefotaxime six weeks after co-cultivation. Fifty to 73% of regenerated shoots showed GUS expression through the histochemical GUS expression analysis. PCR and Southern blot analysis confirmed the existence of npt II and uid A genes in these GUS expressed transformed plants. This is the first report of successful genetic transformation in Asarina procumbens Mill.
引用
收藏
页码:394 / 399
页数:6
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