Selective Perturbation of the BAR Domain of Endophilin Impairs Synaptic Vesicle Endocytosis

被引：15

作者：

Andersson, Fredrik ^{[1
]}

Low, Peter ^{[1
]}

Brodin, Lennart ^{[1
]}

机构：

[1] Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden

来源：

SYNAPSE | 2010年 / 64卷 / 07期

基金：

瑞典研究理事会;

关键词：

clathrin; neurotransmitter release; periactive zone; synapse; DISRUPTION;

D O I：

10.1002/syn.20772

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

The importance of the BAR domain of endophilin in synaptic vesicle endocytosis was tested in presynaptic microinjection experiments in the lamprey giant synapse. Antibodies as well as Fab fragments directed to the BAR domain caused a stimulus-dependent decrease in the number of synaptic vesicles along with an accumulation of shallow clathrin coated pits in the periactive zone. Moreover, the isolated BAR domain protein also caused an accumulation of shallow-coated pits in the periactive zone, in addition to appearance of narrow tubules in synaptic regions. The BAR domain of endophilin is thus required for efficient progression of the synaptic vesicle cycle. Synapse 64:556-560, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：556 / 560

页数：5

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