Comparison of partial gene sequences encoding a phosphoketolase for the identification of bifidobacteria

被引:22
作者
Berthoud, H [1 ]
Chavagnat, F [1 ]
Haueter, M [1 ]
Casey, MG [1 ]
机构
[1] Swiss Fed Res Stn Anim Prod & Dairy Prod, Agroscope Liebefeld Posieux, CH-3003 Bern, Switzerland
关键词
Bifidobacterium; identification; taxonomy; phosphoketolase; xfp;
D O I
10.1016/j.lwt.2004.05.002
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
In this study, a new identification method for Bifidobacterium species based on sequencing of a partial xylulose-5-phosphate/ fructose-6-phosphate phosphoketolase gene (xfp) was evaluated. Approximately 500bp sequences from 68 different strains including 34 type strains were compared. The results showed that this method discriminated between all bifidobacteria, generally with greater accuracy than 16S rDNA sequence analysis. It even clearly distinguished B. longum biovar infantis from B. longum biovar longum and B. longum biovar suis, as well as B. animalis from R lactis. (C) 2004 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:101 / 105
页数:5
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