Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers

被引：154

作者：

Krejci, Alena ^{[1
]}

Bray, Sarah ^{[1
]}

机构：

[1] Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 3DY, England

来源：

GENES & DEVELOPMENT | 2007年 / 21卷 / 11期

基金：

英国医学研究理事会;

关键词：

notch; chromatin; developmental signaling; transcription factor; CSL proteins; E(spl) complex;

D O I：

10.1101/gad.424607

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The CSL [CBF1/Su(H)/Lag2] proteins [Su( H) in Drosophila] are implicated in repression and activation of Notch target loci. Prevailing models imply a static association of these DNA-binding transcription factors with their target enhancers. Our analysis of Su( H) binding and chromatin-associated features at 11 E(spl) Notch target genes before and after Notch revealed large differences in Su( H) occupancy at target loci that correlated with the presence of polymerase II and other marks of transcriptional activity. Unexpectedly, Su( H) occupancy was significantly and transiently increased following Notch activation, suggesting a more dynamic interaction with targets than hitherto proposed.

引用

页码：1322 / 1327

页数：6

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