The efficiency of different IRESs (Internal ribosomes entry site) in monocistronic mRNAS

被引：5

作者：

Attal, J ^{[1
]}

Théron, MC ^{[1
]}

Rival, S ^{[1
]}

Puissant, C ^{[1
]}

Houdebine, LM ^{[1
]}

机构：

[1] INRA, Unite Differenciat Cellulaire, F-78352 Jouy En Josas, France

来源：

MOLECULAR BIOLOGY REPORTS | 2000年 / 27卷 / 01期

关键词：

IRES; translation; monocistronic mRNA;

D O I：

10.1023/A:1007084730470

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The IRES from poliovirus and from encephalomyocarditis virus (EMCV) added between the cap and the AUG initiator codon were strong inhibitors of chloramphenicol acetyltransferase gene expression in three different cell types. The poliovirus IRES also inhibited bGH (bovine growth hormone) cDNA expression in the HC11 mammary cell line when added between the rabbit whey acidic gene promoter and the cDNA whereas the HTLV-1 IRES showed a stimulatory effect in the same situation. RNA stem loops were added before HTLV-1 (SUR) and the BiP (Immunoglobulin heavy-chain Binding Protein) IRESs followed by the firefly luciferase gene under the control of Rous sarcoma virus (RSV) promoter. The RNA loops abolished the expression of the reporter gene almost completely. These data suggest that the different IRESs may favour or inhibit translation of monocistronic mRNA.

引用

页码：21 / 26

页数：6

共 25 条

[1] The RU5 ('R') region from human leukaemia viruses (HTLV-1) contains an internal ribosome entry site (IRES)-like sequence [J].

Attal, J ;

Theron, MC ;

Taboit, F ;

CajeroJuarez, M ;

Kann, G ;

Bolifraud, P ;

Houdebine, LM .

FEBS LETTERS, 1996, 392 (03) :220-224

[2] The optimal use of IRES (internal ribosome entry site) in expression vectors [J].

Attal, J ;

Théron, MC ;

Houdebine, LM .

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING, 1999, 15 (3-5) :161-165

[3] AN INTERNAL RIBOSOMAL ENTRY MECHANISM PROMOTES TRANSLATION OF MURINE LEUKEMIA-VIRUS GAG POLYPROTEIN PRECURSORS [J].

BERLIOZ, C ;

DARLIX, JL .

JOURNAL OF VIROLOGY, 1995, 69 (04) :2214-2222

[4] Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins [J].

Borman, AM ;

LeMercier, P ;

Girard, M ;

Kean, KM .

NUCLEIC ACIDS RESEARCH, 1997, 25 (05) :925-932

[5] INITIATION OF PROTEIN-SYNTHESIS BY THE EUKARYOTIC TRANSLATIONAL APPARATUS ON CIRCULAR RNAS [J].

CHEN, CY ;

SARNOW, P .

SCIENCE, 1995, 268 (5209) :415-417

[6] Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation [J].

Gan, WN ;

Rhoads, RE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (02) :623-626

[7] Internal ribosome entry sites (IRESs): reality and use [J].

Houdebine, LM ;

Attal, J .

TRANSGENIC RESEARCH, 1999, 8 (03) :157-177

[8]

JACKSON RJ, 1990, TIBS, P477

[9] IMPROVED VECTORS FOR STABLE EXPRESSION OF FOREIGN GENES IN MAMMALIAN-CELLS BY USE OF THE UNTRANSLATED LEADER SEQUENCE FROM EMC VIRUS [J].

KAUFMAN, RJ ;

DAVIES, MV ;

WASLEY, LC ;

MICHNICK, D .

NUCLEIC ACIDS RESEARCH, 1991, 19 (16) :4485-4490

[10] ADHERENCE TO THE FIRST-AUG RULE WHEN A 2ND-AUG CODON FOLLOWS CLOSELY UPON THE FIRST (VOL 92, PG 2662, 1995) [J].

KOZAK, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (15) :7134-7134

← 1 2 3 →