Directed evolution and axial chirality:: optimization of the enantioselectivity of Pseudomonas aeruginosa lipase towards the kinetic resolution of a racemic allene

被引：40

作者：

Carballeira, Jose Daniel

Krumlinde, Patrik

Bocola, Marco

Vogel, Andreas

Reetz, Manfred T.

Baeckvall, Jan-E.

机构：

[1] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany

[2] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, SE-10691 Stockholm, Sweden

来源：

CHEMICAL COMMUNICATIONS | 2007年 / 19期

关键词：

D O I：

10.1039/b700849j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Directed evolution of Pseudomonas aeruginosa lipase by the use of combinatorial active site saturation test ( CAST) criteria provided a highly enantioselective mutant (Leu162Phe) for kinetic resolution of an axially chiral allene, p-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate ( E = 111); the high enantioselectivity of the Leu162Phe mutant was rationalized by pi - pi stacking.

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页码：1913 / 1915

页数：3

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