Extracellular metalloproteinase activity in Phytomonas francai

被引：10

作者：

Almeida, FVS

Branquinha, MH

Giovanni-De-Simone, S

Vermelho, AB

机构：

[1] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Dept Microbiol Geral, BR-21941590 Rio De Janeiro, Brazil

[2] Fundacao Oswaldo Cruz, Dept Bioquim & Biol Mol, Lab Bioquim Prot & Peptideos, Rio De Janeiro, Brazil

[3] Univ Fed Fluminense, Dept Biol Celular & Mol, Niteroi, RJ, Brazil

来源：

PARASITOLOGY RESEARCH | 2003年 / 89卷 / 04期

关键词：

Proteolytic Activity; Hemin; Extracellular Proteinase; Ammonium Sulfate Precipitation; Gelatinolytic Activity;

D O I：

10.1007/s00436-002-0792-3

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Extracellular proteolytic activities were detected in Phytonionas francai culture supernatant. A 67-kDa enzyme was purified by ammonium sulfate precipitation and gel filtration in a HPLC system. This proteinase was optimally active at 28 degreesC and pH 5.0; and the use of proteolytic inhibitors indicated that it belongs to the metalloproteinase class. This is the first report on the purification of an extracellular metalloproteinase from a Phytomonas species.

引用

页码：320 / 322

页数：3

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