Efficient multicistronic expression of a transgene in human embryonic stem cells

被引：58

作者：

Hasegawa, Kouichi

Cowan, Aaron B.

Nakatsuji, Norio

Suemori, Hirofumi

机构：

[1] Kyoto Univ, Inst Frontier Med Sci, Lab Embryon Stem Cell Res, Stem Cell Res Ctr,Sakyo Ku, Kyoto 6068507, Japan

[2] Kyoto Univ, Inst Frontier Med Sci, Dept Dev & Differentiat, Sakyo Ku, Kyoto 6068507, Japan

来源：

STEM CELLS | 2007年 / 25卷 / 07期

关键词：

human embryonic stem cells; genetic modification; multicistronic expression;

D O I：

10.1634/stemcells.2006-0813

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The applicability of human embryonic stem cells (hESCs) will be greatly enhanced by techniques that permit efficient genetic modification with multiple transgenes. We report here on single-promoter-driven foot-and-mouth disease virus segment 2A-mediated multicistronic expression of a transgene in hESCs. Efficient multicistronic expression of the transgene was permitted by 2A-mediated separation with almost the same amounts of encoded proteins in hESC. In addition, the multicistronic protein expression was successful in hESC-derived differentiated cells in in vivo and in vitro differentiation assays. This technology may be a significant advance in the genetic engineering of hESCs and hESC-derived cells for purposes that require the reliable expression of multiple transgenes.

引用

页码：1707 / 1712

页数：6

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