Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization

被引:69
作者
Albermann, C
Soriano, A
Jiang, JQ
Vollmer, H
Biggins, JB
Barton, WA
Lesniak, J
Nikolov, DB
Thorson, JS
机构
[1] Univ Wisconsin, Sch Pharm, Pharmaceut Sci Div, Lab Biosynthet Chem, Madison, WI 53705 USA
[2] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA
关键词
D O I
10.1021/ol0341086
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced.
引用
收藏
页码:933 / 936
页数:4
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