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PCR for diagnosis of paracoccidioidomycosis

被引：53
作者：
Gomes, GM
Cisalpino, PS
Taborda, CP
de Camargo, ZP
机构：
[1] Univ Fed Sao Paulo, Disciplina Biol Celular, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil
[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Belo Horizonte, MG, Brazil
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 09期
关键词：
D O I：
10.1128/JCM.38.9.3478-3480.2000
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A PCR assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
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页码：3478 / 3480
页数：3
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