Establishment and Characterization of a Fibroblast Line from Landrace

被引：15

作者：

Bai, Chunyu ^{[1
,2
]}

Li, Chunying ^{[3
]}

Jin, Dapeng ^{[1
]}

Guo, Yu ^{[2
]}

Guan, Weijun ^{[1
]}

Ma, Yuehui ^{[1
]}

Zhao, Qianjun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

[2] Shanxi Agr Univ, Coll Anim Sci & Technol, Taigu, Peoples R China

[3] NE Forestry Univ, Herbin, Peoples R China

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2010年 / 38卷 / 03期

关键词：

CELL-LINES; IDENTIFICATION;

D O I：

10.3109/10731191003670525

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Up to 32 Landrace ear marginal tissue samples were used for establishing a fibroblast cell bank by the means of primary explantation and cryopreservation. Biological analysis suggested that the Population Doubling Time (PDT) of the cell line was approximately 24h. The diploid accounted for 97.2% of the whole population; isozyme analysis of Lactic Dehydrogenase (LDH) and Malic Dehydrogenase(MDH) disproved cross-contamination from other cell lines. The results of bacterium, fungus, virus and mycoplasma tests were all negative. The transfection rates of three fluorescent proteins were high, indicating that the exogenous genes could be effectively expressed in the cells. It had not only preserved the precious germplasm resource of the Landrace on the cell level but also provided valuable material for the researches of genomics, postgenomics, somatic cloning and so on.

引用

页码：129 / 135

页数：7

共 27 条

[1]

[Anonymous], ANIMAL CELLS LIVING

[2]

[Anonymous], ANIMAL CELL CULTURE

[3] Establishment and partial characterization of an ovine synovial membrane cell line obtained by transformation with Simian Virus 40 T antigen [J].

Costa, UM ;

Reischak, D ;

da Silva, J ;

Ravazzolo, AP .

JOURNAL OF VIROLOGICAL METHODS, 2005, 128 (1-2) :72-78

[4]

FRESHNEY RI, 2000, CULTURE ANIMAL CELLS, P49

[5]

Freshney RI., 1994, Culture of animal cells : A manual of basic technique, V3rd, DOI DOI 10.1002/9780470649367

[6]

Guan W.J., 2005, REV CHINA AGR SCI TE, V7, P25

[7]

Holbrook J.J., 1975, The enzymes, P191

[8]

JENKINS N, 1999, ANIMAL CELL BIOTECHN, P132

[9] Establishment and characterization of primary canine hepatocellular carcinoma cell lines producing alpha-fetoprotein [J].

Kawarai, Shinpei ;

Hashizaki, Kazuko ;

Kitao, Satoshi ;

Nagano, Shintaro ;

Madarame, Hiroo ;

Neo, Sakurako ;

Ishikawa, Takefumi ;

Furuichi, Mitsuru ;

Hisasue, Masaharu ;

Tsuchiya, Ryo ;

Tsujimoto, Hajime ;

Yamada, Takatsugu .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2006, 113 (1-2) :30-36

[10] Establishment and characterization of a fibroblast line from Simmental cattle [J].

Li, Lin-feng ;

Yue, Hua ;

Ma, Jianzhang ;

Guan, Wei-jun ;

Ma, Yue-hui .

CRYOBIOLOGY, 2009, 59 (01) :63-68

← 1 2 3 →