Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations

被引：29

作者：

Mellmann, A

Mosters, J

Bartelt, E

Roggentin, P

Ammon, A

Friedrich, AW

Karch, H

Harmsen, D

机构：

[1] Univ Klin Munster, Inst Hyg, D-48149 Munster, Germany

[2] Inst Hyg, Hamburg, Germany

[3] Umwelt, Hamburg, Germany

[4] Bundesinst Risikobewertung, Berlin, Germany

[5] Robert Koch Inst, D-1000 Berlin, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 10期

关键词：

D O I：

10.1128/JCM.42.10.4840-4842.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.

引用

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页码：4840 / 4842

页数：3

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