Stem cell factor and IL-6 do not promote complete maturation of human cultured mast cells from umbilical cord blood cells: an ultrastructural study

被引:9
作者
Matsushima, YR
Ishikawa, O
Kurosawa, M
Miyachi, Y
机构
[1] Gunma Univ, Sch Med, Dept Dermatol, Maebashi, Gumma 3718511, Japan
[2] Hirosaki Univ, Sch Med, Dept Geriatr Med, Hirosaki, Aomori 0368562, Japan
[3] Kyoto Univ, Grad Sch Med, Dept Dermatol, Sakyo Ku, Kyoto 6068507, Japan
关键词
mast cell; cord blood; immunocytochemical staining; electron microscopy; histamine release;
D O I
10.1016/S0923-1811(00)00076-1
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Human mast cells are classified into two phenotypes based on their neutral protease compositions. One type is a tryptase-positive and chymase-positive MCTC cell that is predominant in the skin, another is a tryptase-positive and chymase-negative MC-T cell that is predominant in the lung. Cord blood-derived human mast cells cultured in the presence of stem cell factor and interleukin-6 are a mixture of MCTC and MCT at various ratios, as revealed by immunocytochemical staining. We performed an electron microscopic analysis of cord blood-derived human cultured mast cells and found that they were so immature that we could not distinguish MCT and MCTC from their ultrastructural morphology. The response to secretagogues was not the response of MCTC but rather of MCT. Although human cultured mast cells are the most useful cells for use in in vitro experiments, the present culture condition supplemented with stem cell factor and interleukin-6 does not develop fully mature mast cells in vitro. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:4 / 13
页数:10
相关论文
共 20 条
[1]  
BENYON RC, 1987, J IMMUNOL, V138, P861
[2]  
CHURCH MK, 1982, J IMMUNOL, V129, P2116
[3]  
CRAIG SS, 1989, LAB INVEST, V60, P147
[4]  
CRAIG SS, 1988, LAB INVEST, V58, P682
[5]   HUMAN AND MURINE RECOMBINANT C-KIT LIGANDS SUPPORT THE DEVELOPMENT OF HUMAN MAST-CELLS FROM UMBILICAL-CORD BLOOD-CELLS - ULTRASTRUCTURAL IDENTIFICATION [J].
DVORAK, AM ;
MITSUI, H ;
ISHIZAKA, T .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1993, 101 (03) :247-253
[6]   ULTRASTRUCTURAL MORPHOLOGY OF IMMATURE MAST-CELLS IN SEQUENTIAL SUSPENSION-CULTURES OF HUMAN CORD-BLOOD CELLS SUPPLEMENTED WITH C-KIT LIGAND - DISTINCTION FROM MATURE BASOPHILIC LEUKOCYTES UNDERGOING SECRETION IN THE SAME CULTURES [J].
DVORAK, AM ;
MITSUI, H ;
ISHIZAKA, T .
JOURNAL OF LEUKOCYTE BIOLOGY, 1993, 54 (05) :465-485
[7]   ULTRASTRUCTURAL ANALYSIS OF THE DEVELOPMENT OF HUMAN BASOPHILS AND MAST-CELLS IN-VITRO [J].
DVORAK, AM ;
ISHIZAKA, T .
INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, 1995, 25 (01) :7-24
[8]   ULTRASTRUCTURAL MORPHOLOGY OF HUMAN MAST-CELL PROGENITORS IN SEQUENTIAL COCULTURES OF CORD BLOOD-CELLS AND FIBROBLASTS [J].
DVORAK, AM ;
FURITSU, T ;
ISHIZAKA, T .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1993, 100 (03) :219-229
[9]  
DVORAK AM, 1989, LAB INVEST, V61, P116
[10]  
GILEAD L, 1988, IMMUNOLOGY, V63, P669