Peroxidase catalyzed microbiological oxidation of isosafrol into piperonal

被引：35

作者：

Santos, AS

Pereira, N

da Silva, IM

Sarquis, MIM

Antunes, OAC

机构：

[1] Univ Fed Rio de Janeiro, Inst Quim, Lab 641, BR-21945970 Rio De Janeiro, RJ, Brazil

[2] Fed Univ Para, Dept Engn Quim, Belem, Para, Brazil

[3] Univ Fed Rio de Janeiro, Escola Quim, Dept Engn Bioquim, BR-21945970 Rio De Janeiro, RJ, Brazil

[4] Fiocruz MS, Inst Oswaldo Cruz, Lab Colecao Cultura Fungos, Fiocruz, RJ, Brazil

来源：

PROCESS BIOCHEMISTRY | 2004年 / 39卷 / 12期

关键词：

microbial screening; biotransformation; peroxidase; oxidation; isosafrol; piperonal;

D O I：

10.1016/j.procbio.2003.11.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A detailed and reliable bioassay to detect peroxidase activity in order to select microbial catalytic agents capable to oxidize isosafrol, 4-(1'-propene)-1,2-methylenedioxybenzene, into piperonal, 4-carboxaldehyde-1,2-methylenedioxibenzene is fully described. The systematic methodology was divided into two parts: first peroxidase activity in microbial systems was detected and determined via the guaiacol assay method and secondly, a biotransformation process to oxidize the substrate was developed. Investigation assays to detect peroxidase activities were performed by using UV-Vis spectrometry, while product formation was monitored by gas chromatography. The biooxidative peroxidase catalyzed transformation of isosafrol into piperonal was achieved with 20% conversion. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：2269 / 2275

页数：7

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