共 18 条
Involvement of the terminus of grouper betanodavirus capsid protein in virus-like particle assembly
被引:12
作者:

Lu, MW
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h-index: 0
机构:
Natl Sun Yat Sen Univ, Inst Marine Resources, Dept Marine Resources, Kaohsiung 80424, Taiwan Natl Sun Yat Sen Univ, Inst Marine Resources, Dept Marine Resources, Kaohsiung 80424, Taiwan

Lin, CS
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Sun Yat Sen Univ, Inst Marine Resources, Dept Marine Resources, Kaohsiung 80424, Taiwan Natl Sun Yat Sen Univ, Inst Marine Resources, Dept Marine Resources, Kaohsiung 80424, Taiwan
机构:
[1] Natl Sun Yat Sen Univ, Inst Marine Resources, Dept Marine Resources, Kaohsiung 80424, Taiwan
关键词:
D O I:
10.1007/s00705-002-0901-8
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Dragon grouper, Epinephelus lanceolatus, nervous necrosis virus (DGNNV) consists of 180 copies of capsid protein that encapsulates a bipartite genome of single-stranded (+)RNAs. Expressing the open reading frame (ORF) of RNA2 in Escherichia coli forms virus-like particles (VLPs) that resembles native virus. Deleting N- and C-termini revealed different impacts on VLP formation. Deletion of 35 or 52 residues at the N-terminus completely ruined the VLP assembly, presumably due to removal of positively charged residues for binding RNAs. When deletions were restricted to 4, 16, or 25 N-terminal residues, the assembly of VLPs remained. The ability of VLP formation diminished when 4 to 11 G terminal residues were deleted. The termini that can be deleted without seriously destructing the VLPs are 25 and 3 residues at N- and C-termini, respectively.
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页码:345 / 355
页数:11
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共 18 条
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