Involvement of the terminus of grouper betanodavirus capsid protein in virus-like particle assembly

被引:12
作者
Lu, MW [1 ]
Lin, CS [1 ]
机构
[1] Natl Sun Yat Sen Univ, Inst Marine Resources, Dept Marine Resources, Kaohsiung 80424, Taiwan
关键词
D O I
10.1007/s00705-002-0901-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Dragon grouper, Epinephelus lanceolatus, nervous necrosis virus (DGNNV) consists of 180 copies of capsid protein that encapsulates a bipartite genome of single-stranded (+)RNAs. Expressing the open reading frame (ORF) of RNA2 in Escherichia coli forms virus-like particles (VLPs) that resembles native virus. Deleting N- and C-termini revealed different impacts on VLP formation. Deletion of 35 or 52 residues at the N-terminus completely ruined the VLP assembly, presumably due to removal of positively charged residues for binding RNAs. When deletions were restricted to 4, 16, or 25 N-terminal residues, the assembly of VLPs remained. The ability of VLP formation diminished when 4 to 11 G terminal residues were deleted. The termini that can be deleted without seriously destructing the VLPs are 25 and 3 residues at N- and C-termini, respectively.
引用
收藏
页码:345 / 355
页数:11
相关论文
共 18 条