Mucin-type glycoprotein from Drosophila melanogaster embryonic cells: Characterization of carbohydrate component

被引：41

作者：

Kramerov, AA ^{[1
]}

Arbatsky, NP ^{[1
]}

Rozovsky, YM ^{[1
]}

Mikhaleva, EA ^{[1
]}

Polesskaya, OO ^{[1
]}

Gvozdev, VA ^{[1
]}

Shibaev, VN ^{[1
]}

机构：

[1] ND ZELINSKII INST ORGAN CHEM,MOSCOW 117913,RUSSIA

来源：

FEBS LETTERS | 1996年 / 378卷 / 03期

关键词：

O-glycan; mucin glycoprotein; cultured cell; Drosophila;

D O I：

10.1016/0014-5793(95)01444-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A secreted glycoprotein (GP) with apparent molecular mass of 90 kDa produced by cultured embryonic cells of Drosophila melanogaster was isolated and partially characterized. GP is enriched by Ser + Thr and Pro residues that constitute up to 30% of the total number of amino acids. An abundant carbohydrate moiety (40% of molecular mass) is mainly represented by vertebrate mucin-type O-linked disaccharide units Gal(beta 1-3)-GalNAc, occupying about a half of the total number of Ser + Thr residues and rendering the GP molecule high resistance to protease action. A few of N-glycans are also present in GP. These characteristics allow to consider the Drosophila GP (termed 'mucin-D') as a first representative of invertebrate mucin-type glycoproteins.

引用

页码：213 / 218

页数：6

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