A Multiparallel Bioreactor for the Cultivation of Mammalian Cells in a 3D-Ceramic Matrix

被引：4

作者：

Goralczyk, Vicky ^{[1
]}

Driemel, Gregor ^{[2
]}

Bischof, Andreas ^{[3
]}

Peter, Andrea ^{[1
]}

Berthold, Almuth ^{[4
]}

Kroh, Lothar ^{[2
]}

Blessing, Lucienne ^{[3
]}

Schubert, Helmut ^{[4
]}

King, Rudibert ^{[1
]}

机构：

[1] TU Berlin, Chair Measurement & Control, Berlin, Germany

[2] TU Berlin, Chair Food Chem, Berlin, Germany

[3] TU Berlin, Chair Engn Design & Methodol, Berlin, Germany

[4] TU Berlin, Chair Ceram, Berlin, Germany

来源：

BIOTECHNOLOGY PROGRESS | 2010年 / 26卷 / 02期

关键词：

bioreactor; porosity; cell proliferation; ceramic structure; alumina; PERFUSION BIOREACTOR; TISSUE; SCAFFOLDS; DENSITY; CULTURE; PROLIFERATION; CARTILAGE; SUPPORT; SYSTEM;

D O I：

10.1002/btpr.335

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

For adherently growing cells, cultivation is limited by the provided growth surface. Excellent surface-to-volume ratios are found in highly porous matrices, which have to face the challenge of nutrient supply inside the matrices' caverns. Therefore, perfusion strategies are recommended which often have to deal with the need of developing an encompassing bioreactor periphery. We present a modular bioreactor system based on a porous ceramic matrix that enables the supply of cells with oxygen and nutrients by perfusion. The present version of the reactor system focuses on simple testing of various inoculation and operation modes. Moreover, it can be used to efficiently test different foam structures. Protocols are given to set-up the system together with handling procedures for long-time cultivation of a CHO cell line. Experimental results confirm vital growth of cells inside the matrices caverns. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 26: 556-564, 2010

引用

页码：556 / 564

页数：9

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