Peroxynitrite formed by simultaneous generation of nitric oxide and superoxide selectively inhibits bovine aortic prostacyclin synthase

被引：192

作者：

Zou, MH ^{[1
]}

Ullrich, V ^{[1
]}

机构：

[1] UNIV KONSTANZ,FAC BIOL,D-78434 CONSTANCE,GERMANY

来源：

FEBS LETTERS | 1996年 / 382卷 / 1-2期

关键词：

peroxynitrite; hypochlorite; prostacyclin synthase; ischemia-reperfusion; irreversible inhibition; circulation;

D O I：

10.1016/0014-5793(96)00160-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of various oxidants on bovine aortic prostacyclin synthase was tested with C-14-labelled prostaglandin endoperoxide as substrate, No sensitivity against hydrogen peroxide, superoxide or hydroxyl radicals was observed but hypochlorite inhibited with an IC50 value of 7 mu M. Among the reactive nitrogen species nitric oxide and nitrogen dioxide radicals were ineffective, but peroxynitrite irreversibly blocked prostacyclin biosynthesis with an IC50 value of 50 nM. Peroxynitrite acted within seconds whereas hypochlorite required up to 30 min for completion, Simultaneous generation of nitric oxide and superoxide also caused inhibition which suggested that under pathological conditions like ischemia-reperfusion not only the vasodilatory effects of nitric oxide but also those of prostacyclin could be eliminated.

引用

页码：101 / 104

页数：4

共 17 条

[1] PATHOLOGICAL IMPLICATIONS OF NITRIC-OXIDE, SUPEROXIDE AND PEROXYNITRITE FORMATION [J].