Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis

被引：38

作者：

Garcia-Yoldi, David

Le Fleche, Philippe

Marin, Clara M.

De Miguel, Maria J.

Munoz, Pilar M.

Vergnaud, Gilles

Lopez-Goni, Ignacio

机构：

[1] Univ Navarra, Dept Microbiol & Parasitol, E-31008 Pamplona, Spain

[2] Univ Paris 11, GPMS, Inst Genet & Microbiol, F-91405 Orsay, France

[3] Ctr Etud Bouchet, Div Analyt Microbiol, F-91710 Vert Le Petit, France

[4] Gobierno Aragon, Ctr Invest & Tecnol Agroalimentaria Aragon, Zaragoza, Spain

来源：

VACCINE | 2007年 / 25卷 / 15期

关键词：

Brucella melitensis Rev 1; MLVA; VNTR; genetic stability;

D O I：

10.1016/j.vaccine.2006.09.063

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The assessment of the genetic stability is one of the essential elements to guarantee the biological quality of live anti-bacteria vaccines. Live attenuated Brucella melitensis Rev 1 is the most effective vaccine against brucellosis in small ruminants. Thirty-six B. melitensis Rev 1 vaccine strains isolated from human or animal sources from different geographic regions, from different commercial batches or laboratory collections were typed by the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) recently described for Brucella spp. Our results demonstrated that B. melitensis Rev I group as assayed by MLVA is genetically very homogeneous. We believe that MLVA methodology could be an essential assay to guarantee the quality and stability of live anti-bacterial vaccines being produced worldwide and can be included as in vitro control. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2858 / 2862

页数：5

共 23 条

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