A robust and high-throughput Cre reporting and characterization system for the whole mouse brain

被引：4880

作者：

Madisen, Linda ^{[1
]}

Zwingman, Theresa A. ^{[1
]}

Sunkin, Susan M. ^{[1
]}

Oh, Seung Wook ^{[1
]}

Zariwala, Hatim A. ^{[1
]}

Gu, Hong ^{[1
]}

Ng, Lydia L. ^{[1
]}

Palmiter, Richard D. ^{[2
]}

Hawrylycz, Michael J. ^{[1
]}

Jones, Allan R. ^{[1
]}

Lein, Ed S. ^{[1
]}

Zeng, Hongkui ^{[1
]}

机构：

[1] Allen Inst Brain Sci, Seattle, WA USA

[2] Univ Washington, Dept Biochem, Howard Hughes Med Inst, Seattle, WA 98195 USA

来源：

NATURE NEUROSCIENCE | 2010年 / 13卷 / 01期

基金：

美国国家卫生研究院;

关键词：

GENE-EXPRESSION; TRANSGENIC MICE; SYNAPTIC-TRANSMISSION; FLUORESCENT PROTEIN; DOPAMINE; NEURONS; RECOMBINATION; MANIPULATION; RECEPTORS;

D O I：

10.1038/nn.2467

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The Cre/lox system is widely used in mice to achieve cell-type-specific gene expression. However, a strong and universally responding system to express genes under Cre control is still lacking. We have generated a set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra. The robust native fluorescence of these reporters enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in mapping neuronal circuitry, imaging and tracking specific cell populations in vivo. Using these reporters and a high-throughput in situ hybridization platform, we are systematically profiling Cre-directed gene expression throughout the mouse brain in several Cre-driver lines, including new Cre lines targeting different cell types in the cortex. Our expression data are displayed in a public online database to help researchers assess the utility of various Cre-driver lines for cell-type-specific genetic manipulation.

引用

页码：133 / U311

页数：10

共 48 条

[1] In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2 [J].