Proadrenomedullin N-terminal 20-peptide (PAMP) receptors and signal transduction in the rat adrenal gland

被引：19

作者：

Hinson, JP

Hagi-Pavli, E

Thomson, LM

Kapas, S

机构：

[1] Univ London Queen Mary & Westfield Coll, Fac Basic Med Sci, Dept Biochem, London E1 4NS, England

[2] St Bartholomews & Royal London Sch Med & Dent, Dept Oral Pathol, London E1 2AD, England

来源：

LIFE SCIENCES | 1997年 / 62卷 / 05期

关键词：

PAMP; adrenomedullin; adenylyl cyclase; adrenal cortex; adrenal medulla; zona glomerulosa;

D O I：

10.1016/S0024-3205(97)01137-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Using ligand binding techniques we have demonstrated specific saturable binding of proadrenomedullin N-terminal 20-peptide (PAMP) in the rat adrenal zona glomerulosa. Hill plot analysis revealed two classes of receptor (Kd(1) 1.9nmol/l Bmax 53fmol/mg protein: Kd(2) 10nmol/l, Bmax 225fmol/mg). [I-125]-PAMP was displaced by adrenomedullin at one of these receptors. In the adrenal medulla [I-125]-PAMP bound to a single class of specific receptor (Kd 4.9 nmol/l: Bmax 556fmol/mg). PAMP caused an increase in cAMP generation by zona glomerulosa cells, but not inner zone/medulla cells. These data suggest that PAMP acts through a specific receptor to elevate cAMP levels in the rat adrenal.

引用

页码：439 / 443

页数：5

共 13 条

[1] Effects of adrenomedullin and proadrenomedullin N-terminal 20 peptide on rat zona glomerulosa [J].