Studying biophysical barriers to DNA delivery by advanced light microscopy

被引：41

作者：

De Smedt, SC ^{[1
]}

Remaut, K ^{[1
]}

Lucas, B ^{[1
]}

Braeckmans, K ^{[1
]}

Sanders, NN ^{[1
]}

Demeester, J ^{[1
]}

机构：

[1] Univ Ghent, Dept Pharmaceut, Gen Biochem & Phys Pharm Lab, B-9000 Ghent, Belgium

来源：

ADVANCED DRUG DELIVERY REVIEWS | 2005年 / 57卷 / 01期

关键词：

gene delivery; antisense oligonucleotides; light microscopy; microphotolysis; fluorescence recovery after photobleaching; fluorescence correlation spectroscopy; fluorescence fluctuation spectroscopy; fluorescence resonance energy transfer; DNA degradation;

D O I：

10.1016/j.addr.2004.06.003

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Advanced light microscopy (ALM) has been intensively employed by biophysicists to reveal cellular mechanisms. As described in this review, ALM clearly has potential to enhance our understanding of the mechanisms that affect macromolecular therapeutics or nanoscopic drug vectors in biological environments. However, while in recent years confocal microscopy and related techniques became rather routinely used in drug delivery it remains challenging to extract reliable information on the biophysical behaviour of drug delivery systems from ALM measurements. This review discusses studies in which confocal imaging, fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and fluorescence energy transfer were employed to reveal biophysical properties of DNA and DNA containing nanoparticles in extra- and intracellular media. (C) 2004 Published by Elsevier B.V.

引用

页码：191 / 210

页数：20

共 101 条

[1] Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis [J].

Bacia, K ;

Majoul, IV ;

Schwille, P .

BIOPHYSICAL JOURNAL, 2002, 83 (02) :1184-1193

[2] Direct in vivo measurement of targeted binding in a human tumor xenograft [J].

Berk, DA ;

Yuan, F ;

Leunig, M ;

Jain, RK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (05) :1785-1790

[3] Diffusion and binding measurements within oral biofilms using fluorescence photobleaching recovery methods [J].

Birmingham, JJ ;

Hughes, NP ;

Treloar, R .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1995, 350 (1334) :325-343

[4] FLUORESCENCE PHOTOBLEACHING RECOVERY IN THE CONFOCAL SCANNING LIGHT-MICROSCOPE [J].

BLONK, JCG ;

DON, A ;

VANAALST, H ;

BIRMINGHAM, JJ .

JOURNAL OF MICROSCOPY-OXFORD, 1993, 169 :363-374

[5]

BOUCHER Y, 1990, CANCER RES, V50, P4478

[6] Three-dimensional fluorescence recovery after photobleaching with the confocal scanning laser microscope [J].

Braeckmans, K ;

Peeters, L ;

Sanders, NN ;

De Smedt, SC ;

Demeester, J .

BIOPHYSICAL JOURNAL, 2003, 85 (04) :2240-2252

[7] Encoding microcarriers by spatial selective photobleaching [J].

Braeckmans, K ;

De Smedt, SC ;

Roelant, C ;

Leblans, M ;

Pauwels, R ;

Demeester, J .

NATURE MATERIALS, 2003, 2 (03) :169-173

[8] Encoding microcarriers: Present and future technologies [J].

Braeckmans, K ;

De Smedt, SC ;

Leblans, M ;

Pauwels, R ;

Demeester, J .

NATURE REVIEWS DRUG DISCOVERY, 2002, 1 (06) :447-456

[9] Fluorescence correlation microscopy of cells in the presence of autofluorescence [J].

Brock, R ;

Hink, MA ;

Jovin, TM .

BIOPHYSICAL JOURNAL, 1998, 75 (05) :2547-2557

[10]

Bryers JD, 1998, BIOTECHNOL BIOENG, V60, P462, DOI 10.1002/(SICI)1097-0290(19981120)60:4<462::AID-BIT8>3.0.CO

← 1 2 3 4 5 6 7 8 9 10 →