Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis

被引:100
作者
Chuma, S
Kanatsu-Shinohara, M
Inoue, K
Ogonuki, N
Miki, H
Toyokuni, S
Hosokawa, M
Nakatsuji, N
Ogura, A
Shinohara, T [1 ]
机构
[1] Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Kyoto 6068501, Japan
[2] Kyoto Univ, Dept Dev & Differentiat, Inst Frontier Med Sci, Kyoto 6068507, Japan
[3] Kyoto Univ, Grad Sch Med, Dept Pathol & Biol Dis, Kyoto 6068501, Japan
来源
DEVELOPMENT | 2005年 / 132卷 / 01期
关键词
germ cell; epiblast; rimordial germ cell (PGC); prospermatogonia; spermatogonia; testis; spermatogenesis; transplantation; microinsemination;
D O I
10.1242/dev.01555
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Primordial germ cells (PGCs) are derived from a population of pluripotent epiblast cells in mice. However, little is known about when and how PGCs acquire the capacity to differentiate into functional germ cells, while keeping the potential to derive pluripotent embryonic germ cells and teratocarcinomas. In this investigation, we show that epiblast cells and PGCs can establish colonies of spermatogenesis after transfer into postnatal seminiferous tubules of surrogate infertile mice. Furthermore, we obtained normal fertile offspring by microinsemination using spermatozoa or spermatids derived from PGCs harvested from fetuses as early as 8.5 days post coitum. Thus, fetal male germ cell development is remarkably flexible, and the maturation process. from epiblast cells through PGCs to postnatal spermatogonia, can occur in the postnatal testicular environment. Primordial germ cell transplantation techniques mill also provide a novel tool to assess the developmental potential of PGCs. such as those manipulated in vitro or recovered from embryos harboring lethal mutations.
引用
收藏
页码:117 / 122
页数:6
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