Cloning and characterization of a new member of the T-box gene family

被引：26

作者：

Zhang, WX ^{[1
]}

Yang, SY ^{[1
]}

机构：

[1] New York Med Coll, Immunogenet Mol Lab, Hawthorne, NY 10532 USA

来源：

GENOMICS | 2000年 / 70卷 / 01期

关键词：

D O I：

10.1006/geno.2000.6361

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The T-box is a strongly conserved protein domain, 174 to 186 amino acids in length, that binds DNA. Many genes from many species have been shown to encode T-box domain-containing proteins. Here we report the cloning and characterization of a novel T-box gene, TBX21. The human cDNA contains an open reading frame encoding a 535-amino-acid protein with a predicted molecular mass of 58.3 kDa. Except for the T-box sequence, database searches revealed no significant homology to any known sequences at the nucleotide or protein level. In addition to the human cDNA sequence, we report the cDNA sequence of the murine homologue, the structure and organization of the murine Tbx21 gene, and its localization to mouse chromosome II. TBX21 expression was detected in peripheral blood lymphocytes, spleen, lung, and natural killer cells. (C) 2000 Academic Press.

引用

页码：41 / 48

页数：8

共 27 条

[1]

Agulnik SI, 1996, GENETICS, V144, P249

[2] Cloning, mapping, and expression analysis of TBX15, a new member of the T-box gene family [J].

Agulnik, SI ;

Papaioannou, VE ;

Silver, LM .

GENOMICS, 1998, 51 (01) :68-75

[3]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[4]

Ausubel F.A., 1997, CURRENT PROTOCOLS MO, DOI DOI 10.1.4

[5] Mutations in human TBX3 alter limb, apocrine and genital development in ulnar-mammary syndrome [J].

Bamshad, M ;

Lin, RC ;

Law, DJ ;

Watkins, WS ;

Krakowiak, PA ;

Moore, ME ;

Franceschini, P ;

Lala, R ;

Holmes, LB ;

Gebuhr, TC ;

Bruneau, BG ;

Schinzel, A ;

Seidman, JG ;

Seidman, CE ;

Jorde, LB .

NATURE GENETICS, 1997, 16 (03) :311-315

[6] Mutations in human cause limb and cardiac malformation in Holt-Oram syndrome [J].

Basson, CT ;

Bachinsky, DR ;

Lin, RC ;

Levi, T ;

Elkins, JA ;

Soults, J ;

Grayzel, D ;

Kroumpouzou, E ;

Traill, TA ;

LeblancStraceski, J ;

Renault, B ;

Kucherlapati, R ;

Seidman, JG ;

Seidman, CE .

NATURE GENETICS, 1997, 15 (01) :30-35

[7] AN ANCIENT FAMILY OF EMBRYONICALLY EXPRESSED MOUSE GENES SHARING A CONSERVED PROTEIN MOTIF WITH THE T-LOCUS [J].

BOLLAG, RJ ;

SIEGFRIED, Z ;

CEBRATHOMAS, JA ;

GARVEY, N ;

DAVIDSON, EM ;

SILVER, LM .

NATURE GENETICS, 1994, 7 (03) :383-389

[8]

BUCHBERG AM, 1989, GENETICS, V122, P153

[9] T-BRAIN-1 - A HOMOLOG OF BRACHYURY WHOSE EXPRESSION DEFINES MOLECULARLY DISTINCT DOMAINS WITHIN THE CEREBRAL-CORTEX [J].

BULFONE, A ;

SMIGA, SM ;

SHIMAMURA, K ;

PETERSON, A ;

PUELLES, L ;

RUBENSTEIN, JLR .

NEURON, 1995, 15 (01) :63-78

[10] NUMBER AND EVOLUTIONARY CONSERVATION OF ALPHA-TUBULIN AND BETA-TUBULIN AND CYTOPLASMIC BETA-ACTIN AND GAMMA-ACTIN GENES USING SPECIFIC CLONED CDNA PROBES [J].

CLEVELAND, DW ;

LOPATA, MA ;

MACDONALD, RJ ;

COWAN, NJ ;

RUTTER, WJ ;

KIRSCHNER, MW .

CELL, 1980, 20 (01) :95-105

← 1 2 3 →